Protein Info for GFF3783 in Variovorax sp. SCN45
Annotation: bifunctional P-450:NADPH-P450 reductase 1
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K14338, cytochrome P450 / NADPH-cytochrome P450 reductase [EC: 1.14.14.1 1.6.2.4] (inferred from 93% identity to vpe:Varpa_5460)Predicted SEED Role
"Probable bifunctional P-450/NADPH-P450 reductase CypD"
MetaCyc Pathways
- acetone degradation I (to methylglyoxal) (3/3 steps found)
- acetone degradation III (to propane-1,2-diol) (1/2 steps found)
- 1,5-anhydrofructose degradation (3/5 steps found)
- vanillin biosynthesis I (1/3 steps found)
- melatonin degradation I (2/5 steps found)
- bupropion degradation (1/5 steps found)
- superpathway of melatonin degradation (2/11 steps found)
- nicotine degradation V (3/18 steps found)
- nicotine degradation IV (1/16 steps found)
KEGG Metabolic Maps
- Androgen and estrogen metabolism
- Arachidonic acid metabolism
- Biosynthesis of alkaloids derived from shikimate pathway
- C21-Steroid hormone metabolism
- Caffeine metabolism
- Drug metabolism - cytochrome P450
- Drug metabolism - other enzymes
- Fatty acid metabolism
- Linoleic acid metabolism
- Metabolism of xenobiotics by cytochrome P450
- Retinol metabolism
- Tryptophan metabolism
- gamma-Hexachlorocyclohexane degradation
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.14.14.1 or 1.6.2.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1074 amino acids)
>GFF3783 bifunctional P-450:NADPH-P450 reductase 1 (Variovorax sp. SCN45) MAGKNSLHPIPHPAKKPFVGNLLSIGSDSPVLDMWKIAQDLGGIYWLDMPGMPVIVVSSP ALVDELCEEARFDKSTRGALRRLRAASHGLFTSDTHEETWSKPHNILLANFSQRAMQAYH PMMLDIAGQLVTKWERLNFDEEVDVVRDMTALTLDTIGLCGFGYRFNSFYREGFHPFVDA MVRTLETVQNRRGLPLEELMLKKELAQQRKDIRYMHKMVEDIIEERRASGADIATKPDLL SYMIAGVDKKSGEQLTDKMIRDECIEFLIAGHETTSGLLSFAIYFLLNNPEAMAKAQAEV DNVFGPDTTQKPTYAQVNRLQYVMQVLKESLRLFPTAPAISMRAKEDTKIGGQYTIKKNN MVIMHALALHRDKGIWGENADQFNPDNFSREAERERPVNAFKPFGNGQRACIGRQFALQE AVLTLGMILQRFNLVDHTGYKLKIKEALTIKPENFKIKALLRDPASRPRGNMSDAAAAPV QPAQTAAARKPQAARHGTSLLVLQGSNLGTAEDLARQLAEAGEMRGFSTQLASLDDYAER LPANGAVAIVCASYNGVAPDNAAEFHRWLDKADDALNGVRFSVFGCGNTDWAATYQAVPR RIDERLEALGATRVHPRGEGDAREDMDGAFQDWSDALWPELVKAFDIKTGADTPAQSEPL YTLEELPPPQKNAIVDALGAVALRVIENRELQSGGNDAEAGRSTRHVELMLPEGLVYRAG DHLSVVPRNSPAQVERAMARFGFDRTAHLRLHAVAGRKAALPVEQVIAVDRLLGDYVELQ DVATRKQIATLAAYTECPFTKPKLVALSGSDEASQAAYKAEVLHKRKSLLDLLEEHRACQ VPFAVFLEMLSPLSPRYYSISSSPTMTPGKCSVTVGVVSGPAKSGIGTFEGVCSNFLARA EAGDTVHGVVRETTAEGFRLPEDASRPLIMVGPGTGLAPFRGFLQERAAQVEAGNPQGEA LLFFGCRHPEQDFIYADELQAWAHRGVMKLHTAFSRAGERKVYVQDLIREQSAHVWKLLE AGAVVYVCGDGSRMEPDVRRTLSDLAREHGHDSAAWMDKMIADQRYVLDVWAGS