Protein Info for GFF376 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12) [often with (EC 2.4.99.13) also]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04413: Glycos_transf_N
  • amino acids 11 to 189 (179 residues), 241 bits, E=6.2e-76
• PF00534: Glycos_transf_1
  • amino acids 275 to 373 (99 residues), 26.9 bits, E=3.2e-10

Best Hits

Swiss-Prot: 96% identical to KDTA_ECOL6: 3-deoxy-D-manno-octulosonic acid transferase (waaA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02527, 3-deoxy-D-manno-octulosonic-acid transferase [EC: 2.-.-.-] (inferred from 98% identity to ses:SARI_03916)

MetaCyc: 96% identical to KDO transferase (Escherichia coli K-12 substr. MG1655)
KDOTRANS2-RXN [EC: 2.4.99.13]; KDOTRANS-RXN [EC: 2.4.99.13, 2.4.99.12]

Predicted SEED Role

"Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12) [often with (EC 2.4.99.13) also]" (EC 2.4.99.12, EC 2.4.99.13)

MetaCyc Pathways

• superpathway of Kdo₂-lipid A biosynthesis (23/25 steps found)
• superpathway of (Kdo)₂-lipid A biosynthesis (17/17 steps found)
• Kdo transfer to lipid IV_A (Brucella) (2/2 steps found)
• Kdo transfer to lipid IV_A (E. coli) (2/2 steps found)
• Kdo transfer to lipid IV_A (H. pylori) (2/2 steps found)
• Kdo transfer to lipid IV_A (P. gingivalis) (2/2 steps found)
• Kdo transfer to lipid IV_A (P. putida) (2/2 steps found)
• Kdo transfer to lipid IV_A (generic) (2/2 steps found)
• Kdo transfer to lipid IV_A (Haemophilus) (1/2 steps found)
• Kdo transfer to lipid IV_A (Vibrio cholerae serogroup O1 El Tor) (1/2 steps found)
• Kdo transfer to lipid IV_A (Chlamydia) (3/5 steps found)

KEGG Metabolic Maps

• Lipopolysaccharide biosynthesis
• Puromycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.4.99.12 or 2.4.99.13

Protein Sequence (403 amino acids)

>GFF376 Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12) [often with (EC 2.4.99.13) also] (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
VRGRKAPAYRKRWGERYGFYRRPLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITV
TTMTPTGSERVQSAFGNDVQHVYLPYDLPDALNRFLNKIDPKLVLIMETELWPNLIAALH
KRHIPLVIANARLSARSAAGYAKLGKFVRTLLRRITLIAAQNEEDGERFVALGAKNNQVT
VTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATSTHDGEESIVIAAHQALLHQFPNL
LLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSASTQVVVGDTMGELMLLYGIADLA
FVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICARLDQASGLITITDAATLAKEVS
SLLTDADYRNFYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH