Protein Info for GFF3759 in Sphingobium sp. HT1-2

Annotation: Ubiquinol-cytochrome C reductase, cytochrome B subunit (EC 1.10.2.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 transmembrane" amino acids 46 to 71 (26 residues), see Phobius details amino acids 97 to 119 (23 residues), see Phobius details amino acids 130 to 151 (22 residues), see Phobius details amino acids 158 to 178 (21 residues), see Phobius details amino acids 196 to 218 (23 residues), see Phobius details amino acids 250 to 271 (22 residues), see Phobius details amino acids 292 to 310 (19 residues), see Phobius details amino acids 316 to 334 (19 residues), see Phobius details amino acids 348 to 370 (23 residues), see Phobius details amino acids 376 to 397 (22 residues), see Phobius details PF00033: Cytochrome_B" amino acids 36 to 219 (184 residues), 239.2 bits, see alignment E=5e-75 PF13631: Cytochrom_B_N_2" amino acids 105 to 273 (169 residues), 152.1 bits, see alignment E=2.2e-48 PF00032: Cytochrom_B_C" amino acids 278 to 386 (109 residues), 141.9 bits, see alignment E=1.1e-45

Best Hits

Swiss-Prot: 67% identical to CYB_RHORU: Cytochrome b (petB) from Rhodospirillum rubrum

KEGG orthology group: K00412, ubiquinol-cytochrome c reductase cytochrome b subunit [EC: 1.10.2.2] (inferred from 94% identity to sjp:SJA_C1-30520)

MetaCyc: 53% identical to CYTB cytochrome b (Saccharomyces cerevisiae)

Predicted SEED Role

"Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2)" in subsystem Ubiquinone Menaquinone-cytochrome c reductase complexes (EC 1.10.2.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.10.2.2

Use Curated BLAST to search for 1.10.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (430 amino acids)

>GFF3759 Ubiquinol-cytochrome C reductase, cytochrome B subunit (EC 1.10.2.2) (Sphingobium sp. HT1-2)
MSFPWAEHYTPKHPLMQWVDEKLPLPRLVYNAVGAGYPVPRNLNYFWNFGVLAGLALVIQ
IVTGVVMAMHYGANTLVAFGTVEQTMRDVNAGWLMRYAHANGASFFFIVVYLHIFRGLYF
GSYKAPREMVWLLGLVIFLLMMATAFMGYVLPWGQMSYWGAKVITGLFGAIPVMGEPIQT
WLLGGFAPGNASLNRFFSLHFLLPFVIAAVVILHIWALHIPGSSNPTGVEVKGPQDTVPF
HPYYTAKDGFGAGIFLILFAILLFFAPNYLGHPDNYIEANPLSTPAHIVPEWYFWPFYAI
LRAFTVDFFFVPAKLLGVLAMFASILLLFFLPWLDTSPVRSGSYRPTFKTFFWILVLDVL
ILGYCGGAPAEEPYVMISQVAAAYYFAHFLIILPMIARFEKPLPLPNSITEAVLAKYAKA
DGEPAAVPAE