Protein Info for GFF3758 in Variovorax sp. SCN45
Annotation: NAD-specific glutamate dehydrogenase (EC 1.4.1.2); NADP-specific glutamate dehydrogenase (EC 1.4.1.4)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 58% identical to DHE3_THEMA: Glutamate dehydrogenase (gdhA) from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
KEGG orthology group: K00261, glutamate dehydrogenase (NAD(P)+) [EC: 1.4.1.3] (inferred from 96% identity to vap:Vapar_4808)Predicted SEED Role
"NAD-specific glutamate dehydrogenase (EC 1.4.1.2); NADP-specific glutamate dehydrogenase (EC 1.4.1.4)" (EC 1.4.1.2, EC 1.4.1.4)
MetaCyc Pathways
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- L-glutamate biosynthesis II (1/1 steps found)
- L-glutamate biosynthesis III (1/1 steps found)
- L-glutamate degradation I (1/1 steps found)
- L-glutamate degradation X (1/1 steps found)
- GABA shunt I (3/4 steps found)
- 4-aminobutanoate degradation V (5/7 steps found)
- L-alanine degradation II (to D-lactate) (2/3 steps found)
- ethene biosynthesis IV (engineered) (1/3 steps found)
- L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
- methylaspartate cycle (12/19 steps found)
- L-glutamate degradation XI (reductive Stickland reaction) (3/7 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- D-Glutamine and D-glutamate metabolism
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.4
Use Curated BLAST to search for 1.4.1.2 or 1.4.1.3 or 1.4.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (424 amino acids)
>GFF3758 NAD-specific glutamate dehydrogenase (EC 1.4.1.2); NADP-specific glutamate dehydrogenase (EC 1.4.1.4) (Variovorax sp. SCN45) MSEKLSFVSPTANSPWGTYLSQVDRVVPYLGPLARWVETLKRPKRALIVDVPIEMDDGTI AHFEGYRVQHNMSRGPGKGGVRFHPDVTLEEVMALSAWMTIKTAAVNLPYGGAKGGIRVD PKKLSLQELEKVTRRYTSEIGIIIGPHTDIPAPDVNTNAQIMAWMMDTYSMNVGGTATGV VTGKPLHLGGSLGRVKATGRGVFVTGREAARRLGMDLRGARIAVQGFGNVGSVAAELFVE AGAKVVAVQDHTGTIVNSNGLDLAKLIPVANKEGVVAFKAGGDIVPNEDFWNTPCDILIP AALEGQLTAERAQKTTAKLVLEGANGPTVPQADDILAERGVLVVPDVICNAGGVTVSYFE WVQDFSSFFWDEDEINVRLDRIMMNALNQIWDTADKHKIPLRTATYAVACERILMARQER GLYP