Protein Info for GFF3757 in Xanthobacter sp. DMC5

Annotation: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-di aminopimelate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 492 transmembrane" amino acids 236 to 247 (12 residues), see Phobius details TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 25 to 487 (463 residues), 430.1 bits, see alignment E=6.1e-133 PF01225: Mur_ligase" amino acids 26 to 73 (48 residues), 35.5 bits, see alignment 1.6e-12 PF08245: Mur_ligase_M" amino acids 112 to 313 (202 residues), 131.9 bits, see alignment E=4.7e-42 PF02875: Mur_ligase_C" amino acids 335 to 463 (129 residues), 109.4 bits, see alignment E=3.4e-35

Best Hits

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 83% identity to xau:Xaut_1849)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (492 amino acids)

>GFF3757 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-di aminopimelate ligase (Xanthobacter sp. DMC5)
MTMHSLGQLLDGHARILTPDGAEEPITGLSSDSRRITPGDLFVAIPGTHADGTRFIADAI
ARGAAAVLMEPTAPAPILPPQAALAFTLDARRSLSLAAARVYPRQPQTIAAITGTAGKTS
VAFFLRQIWERLGHRAAYLGTIGLVKPNGATYGSLTTPDPVELHATLDALAGEGVTHMAL
EASSHGLDQKRLDGVRLSAGGFTNLGRDHLDYHPTMDAYFRAKLRLFTEVMPLDAAAVAV
TSAPFAATALAWAHTRGLRLLPIGEGRADGIDIAAATGSPAGQKVMFADGTSAVVPLVGR
FQLDNALLAAGLAIACGADRTEAFAALAHLKGVPGRLERIGDDPRRPVFVDYAHKPEALA
TVLDTLRAAIPGRLVVVVGCGGDRDRGKRPIMGAIAAMKADTVIVTDDNPRSEDPAAIRA
EVLEGARGAGIGNVREIGDRAEAIRAGVALIESGDALLVAGKGHETGQIIGDRTYPFSDA
AEVLAALKEVAA