Protein Info for GFF3743 in Variovorax sp. SCN45

Annotation: Twitching motility protein PilT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 TIGR01420: twitching motility protein" amino acids 3 to 343 (341 residues), 502.2 bits, see alignment E=3.9e-155 PF00437: T2SSE" amino acids 10 to 270 (261 residues), 167.4 bits, see alignment E=1.8e-53

Best Hits

Swiss-Prot: 75% identical to PILT_PSEAE: Twitching mobility protein (pilT) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02669, twitching motility protein PilT (inferred from 99% identity to vpe:Varpa_5474)

Predicted SEED Role

"Twitching motility protein PilT" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (347 amino acids)

>GFF3743 Twitching motility protein PilT (Variovorax sp. SCN45)
VDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHR
KHYEEFLEVDFSFEIDGLARFRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELAL
KPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVEDPIEFVHESKKCLINQREVGP
MTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQSRVAAHEIMLGTPAIRNLIREAKVA
QMYSTIQTGQGQGMQTLDQNLTELVRRNTISAAEARGKAKIPENFPG