Protein Info for GFF3742 in Xanthobacter sp. DMC5

Annotation: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 625 TIGR00136: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA" amino acids 10 to 617 (608 residues), 807.6 bits, see alignment E=3.3e-247 PF12831: FAD_oxidored" amino acids 11 to 181 (171 residues), 40.6 bits, see alignment E=6.3e-14 PF00890: FAD_binding_2" amino acids 11 to 47 (37 residues), 22.1 bits, see alignment 2.4e-08 PF01134: GIDA" amino acids 11 to 399 (389 residues), 552.5 bits, see alignment E=1.7e-169 PF21680: GIDA_C_1st" amino acids 463 to 553 (91 residues), 69.8 bits, see alignment E=8.2e-23 PF13932: SAM_GIDA_C" amino acids 560 to 613 (54 residues), 75.8 bits, see alignment 5.7e-25

Best Hits

Swiss-Prot: 81% identical to MNMG_AZOC5: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K03495, glucose inhibited division protein A (inferred from 87% identity to xau:Xaut_1834)

Predicted SEED Role

"tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (625 amino acids)

>GFF3742 tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (Xanthobacter sp. DMC5)
MNATPHPLAFDVIVVGGGHAGCEAAAAAARVGARTALVTHAQATIGAMSCNPAIGGLGKG
HLVREIDALDGLMGRVADQGGIQFRVLNRRKGPAVRGPRAQADRKLYARAMQQALAEQPG
LEVVEAEADDLLVKDGRVVGLRLADRRELACGAVVLTTGTFLNGLIHLGEESYPAGRMGE
KPALGLSPALSRLGFALGRLKTGTPARLDGRTIDWASLEMQAGDDPPEPFSALTTRLPNP
QVECGITRTTDATHELIRQNLSRSAMYSGRIGSTGPRYCPSIEDKIVRFGDRDGHQIFLE
PEGLDDPTVYPNGLSTSLPADVQLAFLRTIPGLEQVAVLRPGYAIEYDHVDPRELAPTLE
TKRVAGLFLAGQINGTTGYEEAAAQGLLAGLNAARRAGGSDGIVLDRAEAYLGVMVDDLV
TRGVSEPYRMFTSRAEYRLTLRADNADQRLTARGDALGLVGAERLAHFHARTQALEGARA
LTCNLSLTPTEGARHGLALNRDGQRRSAFDLLAYPDIDMARLAMIWPELSALPTAIAEQV
EIDAKYAVYLERQAADIHTLRKDEALSLPEELSYADLPGLSNELKQKLERVRPRTVGQAG
RMEGMTPAALALIATHARRAGGRPA