Protein Info for GFF3739 in Variovorax sp. SCN45

Annotation: 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 302 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details PF02826: 2-Hacid_dh_C" amino acids 8 to 114 (107 residues), 29.7 bits, see alignment E=1.2e-10 PF01488: Shikimate_DH" amino acids 9 to 83 (75 residues), 29.5 bits, see alignment E=2.2e-10 PF03446: NAD_binding_2" amino acids 16 to 176 (161 residues), 174.1 bits, see alignment E=7.1e-55 PF03807: F420_oxidored" amino acids 16 to 81 (66 residues), 40.5 bits, see alignment E=1e-13 PF14833: NAD_binding_11" amino acids 181 to 299 (119 residues), 123.2 bits, see alignment E=2.3e-39

Best Hits

Swiss-Prot: 66% identical to Y1503_SHEFN: Uncharacterized oxidoreductase Sfri_1503 (Sfri_1503) from Shewanella frigidimarina (strain NCIMB 400)

KEGG orthology group: None (inferred from 93% identity to vpe:Varpa_5476)

MetaCyc: 35% identical to tartronate semialdehyde reductase (Escherichia coli K-12 substr. MG1655)
2-hydroxy-3-oxopropionate reductase. [EC: 1.1.1.60]

Predicted SEED Role

"2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Photorespiration (oxidative C2 cycle) (EC 1.1.1.60)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.60

Use Curated BLAST to search for 1.1.1.60

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (302 amino acids)

>GFF3739 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60) (Variovorax sp. SCN45)
MSSINTRTYESVPAQTVAFLGLGVMGGPMAGHLAKAGHAVTVYNRTPAKSQAWAAEFGAP
GRHAPTPREAAAGADIVFCCVGNDDDLRSVVLGDDGAFAGMKKGAAFVDHTTASADVARE
LSKAAQALGLHFIDAPVSGGQAGAQNGALTVMCGGDAAVFERIKPVAMAFSRAVTLLGES
GAGQLAKMVNQIAIAGLVQGLSEAIAFGQRAGLDMEAVLGVISKGAAQSWQMENRGKTMI
DGKFDYGFAVDWMRKDLGLVLDEAKRNGARVPVTALVDQFYADVQQAGGRRWDTSSLITR
LK