Protein Info for GFF3737 in Xanthobacter sp. DMC5

Annotation: Shikimate dehydrogenase (NADP(+))

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00507: shikimate dehydrogenase
  • amino acids 6 to 265 (260 residues), 211.4 bits, E=6.8e-67
• PF08501: Shikimate_dh_N
  • amino acids 7 to 89 (83 residues), 82.6 bits, E=3.8e-27
• PF01488: Shikimate_DH
  • amino acids 122 to 192 (71 residues), 47.3 bits, E=4.8e-16
• PF18317: SDH_C
  • amino acids 240 to 262 (23 residues), 30 bits, see alignment (E = 6.7e-11)

Best Hits

Swiss-Prot: 61% identical to AROE_AZOC5: Shikimate dehydrogenase (NADP(+)) (aroE) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K00014, shikimate dehydrogenase [EC: 1.1.1.25] (inferred from 80% identity to xau:Xaut_1829)

Predicted SEED Role

"Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 1.1.1.25)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• superpathway of chorismate metabolism (41/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.25

Use Curated BLAST to search for 1.1.1.25

Protein Sequence (282 amino acids)

>GFF3737 Shikimate dehydrogenase (NADP(+)) (Xanthobacter sp. DMC5)
MTVRACVTGQPVTYSRSPLLHGYWLKAYGIDGAYEREEVPPEMAADFYRSLAARGYAGCN
VTLPNKEIAFSVLDEADESAAALGAANTLWLDGGKLHGASTDGYGFIANLDASAPGWDGA
RNCALVVGAGGASRAVVHALVGRGFDRVVLANRTLERAETVASLFGPRVRPLAFDRIAEV
MGEADILVNCTSLGMKGSDPLTLDLTRLRQDAVVSDIVYVPLMTPLLKAASAAGFRTVDG
LGMLLHQGVPGFRRWFGVTPEVTRELRAVLVDDLRAKGQLEA