Protein Info for PS417_19130 in Pseudomonas simiae WCS417
Updated annotation (from data): L-lactate dehydrogenase (cytochrome) (EC 1.1.2.3)
Rationale: Specifically important for utilizing Sodium L-Lactate. Automated validation from mutant phenotype: the predicted function (1.1.2.3) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: lactate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 97% identical to LLDD_PSEFS: L-lactate dehydrogenase (lldD) from Pseudomonas fluorescens (strain SBW25)
KEGG orthology group: K00101, L-lactate dehydrogenase (cytochrome) [EC: 1.1.2.3] (inferred from 97% identity to pfs:PFLU4305)MetaCyc: 76% identical to L-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
1.1.5.M6 [EC: 1.1.5.M6]; RXN0-7227 [EC: 1.1.5.M6]
Predicted SEED Role
"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)
MetaCyc Pathways
- L-lactaldehyde degradation (aerobic) (1/2 steps found)
- methylglyoxal degradation IV (1/3 steps found)
- methylglyoxal degradation V (1/3 steps found)
- lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) (4/8 steps found)
- superpathway of methylglyoxal degradation (4/8 steps found)
- superpathway of fucose and rhamnose degradation (4/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.2.3
Use Curated BLAST to search for 1.1.2.3 or 1.1.5.M6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7UEV1 at UniProt or InterPro
Protein Sequence (376 amino acids)
>PS417_19130 L-lactate dehydrogenase (cytochrome) (EC 1.1.2.3) (Pseudomonas simiae WCS417) MIISSASDYRAAAKRKLPRFLFDYIDGGAYAEHTLRANSSDLAEISLRQRILRNVDNLSL KTTLFGQELDMPVILSPVGLTGMYARRGEVQAAKAAANKGIPFCLSTVSVCPIEEVASQS AQAIWFQLYVLKDRGFMRNALERAQAAGVTTLVFTVDMPTPGARYRDAHSGMSGPFAAQR RMLQAVTKPQWAFDVGLMGRPHDLGNISKYLGKPTHLEDYIGWLANNFDASISWKDLEWI REFWKGPMIIKGILDPQDAKDAVSFGADGIVVSNHGGRQLDGVLSTAKALPPIADAVGDD LTVLVDSGIRSGLDVVRMLALGAKACLLGRATAYALAADGQHGVENLLDIFAKEMRVAMT LTGVTSIAQIDRSTLV