Protein Info for GFF3724 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Acetoacetyl-CoA synthetase [leucine] (EC 6.2.1.16)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Predicted SEED Role
"Acetoacetyl-CoA synthetase [leucine] (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 6.2.1.16)
MetaCyc Pathways
- superpathway of fatty acids biosynthesis (E. coli) (48/53 steps found)
- superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
- palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
- palmitate biosynthesis III (21/29 steps found)
- 2-deoxy-D-ribose degradation II (4/8 steps found)
- sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
- stearate biosynthesis I (animals) (1/6 steps found)
- ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.16
Use Curated BLAST to search for 6.2.1.16
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (531 amino acids)
>GFF3724 Acetoacetyl-CoA synthetase [leucine] (EC 6.2.1.16) (Hydrogenophaga sp. GW460-11-11-14-LB1) MALATLHTIADLVEALARERGDSPALTFIDHAAPLAQGTEHRTYAQLWDAATRLATGLSR QGFGPGDRAALLMANHPAFVELLIASSLIGLTLVPIDPRTPPERLAFMLSKTRCALLVAE PTALPSALAVRYTCPSLRLIAVLGEPPSPSAVTYDSLMADTASPDIAAQRRPADEDMQIL FTSGTTGNPKGIVYSHRKFTDTARMAASAFGYRPDDRLYSGLSLTHANAQLVTLGAALVR GLPVVFSRRFSKSRMWQTVREHRCTSLTLLGGMTTAVYCAPPTPADRDHQVRLVVSAGMP ATIWSDFEHRFGVSLLEFYGTAEGGLTMKPPGQGPAGSIGQPPPTLSIRIVDDAGQDVAP GERGQLLIRSADGTPFQVAYVDEPEASQAKCRDGWLWTGDVVHRDADGWLFFDFRMGHGI RRNGDFIDTAFIEKTLAETGLVDDVFVFGVPAASRVPGESDVAAAVVFAQNAPADQGLAV AALRAALPRPVVLQQVLPLKAIPKTASEKPLARVLSQRLAEQPGLALDWPA