Protein Info for GFF3716 in Variovorax sp. SCN45

Annotation: Possible hydrolase or acyltransferase RutD in novel pyrimidine catabolism pathway

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 268 TIGR03611: pyrimidine utilization protein D" amino acids 4 to 259 (256 residues), 354.6 bits, see alignment E=1.5e-110 PF12146: Hydrolase_4" amino acids 16 to 241 (226 residues), 83.9 bits, see alignment E=1.6e-27 PF00561: Abhydrolase_1" amino acids 17 to 133 (117 residues), 79.8 bits, see alignment E=3.9e-26 PF12697: Abhydrolase_6" amino acids 18 to 251 (234 residues), 68.8 bits, see alignment E=1.6e-22

Best Hits

Swiss-Prot: 82% identical to RUTD_VARPS: Putative aminoacrylate hydrolase RutD (rutD) from Variovorax paradoxus (strain S110)

KEGG orthology group: K09023, protein RutD (inferred from 82% identity to vap:Vapar_4841)

Predicted SEED Role

"Possible hydrolase or acyltransferase RutD in novel pyrimidine catabolism pathway" in subsystem Pyrimidine utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (268 amino acids)

>GFF3716 Possible hydrolase or acyltransferase RutD in novel pyrimidine catabolism pathway (Variovorax sp. SCN45)
MPSLHYEVHGPSDAPSTVLLSSGLGGSAGFWQPQLGALIAAGHRVVAYDQRGTGRSPAAL
PEAYAIEDMARDVLEILDATRTAQCHFAGHALGGLVGLRLALDAPARVASLVLVNAWSKP
NPHSARCFDARLALLGAVGPRAYVEAQPIFLYPAAWCAAHAKRVADEVDHAFAHFPGEAN
MRARIAALRAFDIDARLGDITAPTLVAAAKDDVLVPWTCSQRLADELPNATLNLMAHGGH
AHSVTEADAFNSTLLAFLSRVDAPAVTA