Protein Info for GFF3704 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 835 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 1 to 789 (789 residues), 1344.6 bits, see alignment E=0 PF07992: Pyr_redox_2" amino acids 19 to 293 (275 residues), 179.3 bits, see alignment E=4.1e-56 PF13738: Pyr_redox_3" amino acids 71 to 268 (198 residues), 37.5 bits, see alignment E=6e-13 PF00070: Pyr_redox" amino acids 136 to 215 (80 residues), 66.5 bits, see alignment E=8.6e-22 PF18267: Rubredoxin_C" amino acids 309 to 375 (67 residues), 38.3 bits, see alignment E=3.7e-13 PF04324: Fer2_BFD" amino acids 412 to 459 (48 residues), 55.1 bits, see alignment 2.7e-18 PF03460: NIR_SIR_ferr" amino acids 548 to 610 (63 residues), 42 bits, see alignment 2.2e-14 PF01077: NIR_SIR" amino acids 621 to 756 (136 residues), 69.6 bits, see alignment E=7.8e-23

Best Hits

Swiss-Prot: 95% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 98% identity to cro:ROD_44441)

MetaCyc: 95% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (835 amino acids)

>GFF3704 Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MVGHRFIEDLLDKSDASLFDITVFCEEPRKAYDRVHLSSYFSHHTAEELSLVREGFYEKH
GVKVLVGERAITINRQEKVIHSSAGRTVYYDKLIMATGSYPWIPPIKGSETQDCFVYRTI
EDLNAIEACARRSKRGAVVGGGLLGLEAAGALKNLGVETHVIEFAPMLMAEQLDQMGGEQ
LRRKIESMGVRVHTSKNTQEIVQQGNEARKTMRFADGSELEVDFIVFSTGIRPRDKLATQ
CGLAVAQRGGIVINDTCQTSDPDIYAIGECASWNNRVYGLVAPGYKMAQVAVDHILGSEN
AFEGADLSAKLKLLGVDVGGIGDAHGRTPGARSYVYLDESKEVYKRLIVSEDNKTLLGAV
LVGDTSDYGNLLQLVLNAIELPENPDSLILPSHAGSGKPAIGVDKLPDSAQICSCFDVTK
GDLIAAINKGCHTVAALKAETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYS
RQELFHLIRVEGIKTFEELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPQHTPLQDTN
DNFLANIQKDGTYSVIPRSAGGEITPEGLVAVGRIAREFNLYTKITGSQRIGLFGAQKDD
LPEIWRQLIEAGFETGHAYAKALRMAKTCVGSTWCRYGVGDSVGFGVELENRYKGIRTPH
KMKFGVSGCTRECAEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADLDRDTLIKY
LDRFMMFYIRTADKLTRTAPWLDNLEGGIEYLKSVIIDDKLGLNEHLEEEMARLRAAVVC
EWTETVNTPSAQVRFKHFINSDKRDPNVQVVPEREQHRPATPYERIPVTLVEENA