Protein Info for GFF3698 in Variovorax sp. SCN45

Annotation: ATP-dependent, 3'-5' DNA helicase with strand annealing activity

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 918 TIGR04121: DEXH box helicase, DNA ligase-associated" amino acids 56 to 872 (817 residues), 1109.7 bits, see alignment E=0 PF00270: DEAD" amino acids 69 to 249 (181 residues), 91.5 bits, see alignment E=1.3e-29 PF04851: ResIII" amino acids 73 to 244 (172 residues), 28.4 bits, see alignment E=3.6e-10 PF00271: Helicase_C" amino acids 296 to 403 (108 residues), 49.9 bits, see alignment E=8.7e-17 PF19306: WH_Lhr" amino acids 430 to 594 (165 residues), 215.2 bits, see alignment E=9.5e-68 PF08494: DEAD_assoc" amino acids 647 to 843 (197 residues), 162.1 bits, see alignment E=3.6e-51

Best Hits

KEGG orthology group: K03724, ATP-dependent helicase Lhr and Lhr-like helicase [EC: 3.6.4.-] (inferred from 89% identity to vpe:Varpa_5497)

Predicted SEED Role

"FIG003033: Helicase domain protein"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (918 amino acids)

>GFF3698 ATP-dependent, 3'-5' DNA helicase with strand annealing activity (Variovorax sp. SCN45)
MSTKPEPEDFLPPLPGEGRGGGRRRIEDPLSTEGRLPPSQPSPSGGRSKTKVKAALDAWF
AERGWKPFKFQRDVWKAIAQGRSGLLHATTGAGKTYAVWLGALQAFSLPRKETARPAPPP
LTVLWLTPMRALAADTLRALQQPLEALGAEVHPWSTGARSGDTSSAERSAQNQRLPSVLV
TTPESLSLLLARADANEVLGSVRLAVVDEWHELLGNKRGVQVQLALARLRRWNPGMTVWG
MSATLGNLKEAMHALLGHEEGVLVQGQVPKKLVIDSLLPGRAERFPWGGHLGLTMLPQVI
EEIAASKTTLVFTNTRSQSEIWYQALLEARPEWAGSIALHHGSLDREVREWVELGLKSGE
LKAVVCTSSLDLGVDFLPVERVLQIGSPKGVARLLQRAGRSGHAPGRPSRITLVPTHSIE
MVEGAAARAAIAEGHIEARHSPDQPLDVLVQHLVTVALGGGFLPDDLYAEVRGTAAYENL
SRESWQWCLDFVSQGGPSLSAYPDYRRAVPDAEGVWRVPDARLARRHRMNIGTIVSDASM
SVQFVGGAKIGSVEESFVARMKPGDCFLFGGRLLELVRIHDMTAWVRRASGKRPAVPRWN
GGRMPLSNTLADAVVRQLALAGDGAYDSPELQCVRPLLEIQQQWSALPTPHTLLAETLAT
REGSHLFLYPFAGRHVHLGLASLLAWRVAQHEARTFSIAVNDYGFELLSATEVDWPALLP
QVLRLPEGNGEAGGADAALLHEVLASLNAGELAQRRFREIARVSGLVFQGYPGEKRSSRQ
LQASSSLFWEVFRKYDPDNRLLQQAEQELLAQELEIGRLRASLVRMAGQQLVLKPLARPT
PFSFPLMVELFREKLSNESVADRIARMVEQLEKAAGGAVTAGGVERVKSTMAFGQEGAGK
PPAPRRERRRPSRPLPPL