Protein Info for GFF3692 in Pseudomonas sp. DMC3

Annotation: Sulfite reductase [ferredoxin]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03460: NIR_SIR_ferr
  • amino acids 53 to 109 (57 residues), 58.8 bits, 3.5e-20
  • amino acids 343 to 398 (56 residues), 43.5 bits, 2.2e-15
• PF01077: NIR_SIR
  • amino acids 119 to 273 (155 residues), 163.3 bits, E=3.2e-52
  • amino acids 411 to 547 (137 residues), 52.8 bits, E=3.2e-18

Best Hits

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 98% identity to pfo:Pfl01_3112)

MetaCyc: 46% identical to assimilatory sulfite reductase (ferredoxin) (Allochromatium vinosum)
Sulfite reductase (ferredoxin). [EC: 1.8.7.1]

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction IV (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• assimilatory sulfate reduction II (2/3 steps found)

KEGG Metabolic Maps

• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2 or 1.8.7.1

Protein Sequence (552 amino acids)

>GFF3692 Sulfite reductase [ferredoxin] (Pseudomonas sp. DMC3)
MYVYDEYDQRIIEDRVKQFRDQTRRYLAGELSEEEFRPLRLQNGLYIQRFAPMLRVAVPY
GQLTSRQVRMMAKIARDYDKGYAHISTRQNVQFNWPAVEDIPDILAELATVQMHAIQTSG
NCLRNVTTDQFAGVAADEVIDPRPWCEIVRQWTTFHPEFAYLPRKFKIAVNGSTSDRAAI
EVHDIGLEPVYNAAGELGFRVLVGGGLGRTPVVGAFINEFLPWQDLLSYLDAILRVYNRY
GRRDNKYKARIKILVKALTPEVFAQKVDAEMEHLRGGQTTLTEAELHRVAKHFVDPDYKT
LDNQTAQLADLDQEHPGFARWRSRNTLAHKKPGYVAVTLSLKPTGVAPGDITDKQLDAVA
DLAERYSFGQLRTSHEQNIILADVEQSQLFTLWGELREGGFATPNIGLLTDIICCPGGDF
CSLANAKSIPIAESIQRRFDDLDYLFDIGELDLNISGCMNACGHHHVGHIGILGVDKKGE
EFYQVSLGGSASRDASLGKILGPSFAQEAMPDVISKLIDVYVEQRTEDERFIDTYQRIGI
DLFKERVYAANH