Protein Info for GFF369 in Variovorax sp. SCN45

Annotation: Cell division-associated, ATP-dependent zinc metalloprotease FtsH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 638 transmembrane" amino acids 6 to 23 (18 residues), see Phobius details amino acids 106 to 128 (23 residues), see Phobius details PF06480: FtsH_ext" amino acids 11 to 101 (91 residues), 48.3 bits, see alignment E=3.2e-16 TIGR01241: ATP-dependent metallopeptidase HflB" amino acids 109 to 600 (492 residues), 791.4 bits, see alignment E=1.8e-242 PF07728: AAA_5" amino acids 196 to 310 (115 residues), 23 bits, see alignment E=2e-08 PF00004: AAA" amino acids 197 to 329 (133 residues), 160.5 bits, see alignment E=8.8e-51 PF17862: AAA_lid_3" amino acids 352 to 396 (45 residues), 56.3 bits, see alignment 5.3e-19 PF01434: Peptidase_M41" amino acids 410 to 599 (190 residues), 244.3 bits, see alignment E=2.8e-76

Best Hits

Swiss-Prot: 74% identical to FTSH_CUPMC: ATP-dependent zinc metalloprotease FtsH (ftsH) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K03798, cell division protease FtsH [EC: 3.4.24.-] (inferred from 98% identity to vap:Vapar_2624)

MetaCyc: 64% identical to ATP-dependent zinc metalloprotease FtsH (Escherichia coli K-12 substr. MG1655)
3.4.24.-

Predicted SEED Role

"Cell division protein FtsH (EC 3.4.24.-)" in subsystem Bacterial Cell Division (EC 3.4.24.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (638 amino acids)

>GFF369 Cell division-associated, ATP-dependent zinc metalloprotease FtsH (Variovorax sp. SCN45)
LNNQWFSKVAVWLVIAMVLFTVFKQFDTRGGVGSGAVSYSEFLDQVRNNQIKSAVIPEGA
AGGEIVAVTNDDRKIRTTATVLDRGLVGDLIDHNVKFDVKPREEGSLLMTLLVSWGPMLL
LIGVWIYFMRQMQGGGKGGAFSFGKSKARMMDENNNTVTFADVAGCDEAKEEVREVVDFL
KDPQRFQKLGGRIPRGLLLVGPPGTGKTLLAKSIAGEAKVPFFSISGSDFVEMFVGVGAA
RVRDMFENAKKNAPCIIFIDEIDAVGRQRGAGLGGGNDEREQTLNQMLVEMDGFETNLGV
IVVAATNRPDILDAALLRPGRFDRQVYVTLPDIRGREQILGVHMRKVPLGQDVNPSVIAR
GTPGMSGADLANLCNEAALMAARRNARVVEMQDFEKAKDKIFMGPERKSMVMPEEERRNT
AYHESGHALIGKLLPKCDPVHKVTIIPRGRALGVTMSLPSQDRYSYDREYMLNQISMLFG
GRIAEEVFMHQMTTGASNDFERATSIARDMVTRYGMTDALGPMVYAENEGEVFLGRSVTK
TTNMSEQTMEKVDSEVRRIIDEQYALARSLIEENSDKMHAMAKALLEWETIDSEQLDDIM
AGRAPRPPKDWTPRIPPSGSGGSGGTPAVNPDPAPTAA