Protein Info for GFF3689 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Asparaginyl-tRNA synthetase (EC 6.1.1.22)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 445 TIGR00457: asparagine--tRNA ligase" amino acids 1 to 445 (445 residues), 685.8 bits, see alignment E=1.4e-210 PF01336: tRNA_anti-codon" amino acids 1 to 78 (78 residues), 48.7 bits, see alignment E=5.8e-17 PF00152: tRNA-synt_2" amino acids 103 to 439 (337 residues), 228.7 bits, see alignment E=1e-71

Best Hits

Swiss-Prot: 100% identical to SYN_SALTY: Asparagine--tRNA ligase (asnS) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01893, asparaginyl-tRNA synthetase [EC: 6.1.1.22] (inferred from 100% identity to spq:SPAB_02511)

MetaCyc: 94% identical to asparagine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Asparagine--tRNA ligase. [EC: 6.1.1.22]

Predicted SEED Role

"Asparaginyl-tRNA synthetase (EC 6.1.1.22)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.22

Use Curated BLAST to search for 6.1.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (445 amino acids)

>GFF3689 Asparaginyl-tRNA synthetase (EC 6.1.1.22) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
VRGWVRTRRDSKAGISFLAVYDGSCFDPVQAVINNSLPNYNEEVLHLTTGCSVVVTGKVV
ASPGQGQSFEIQATKVEVAGWVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVR
HTLAQALHRFFDEQGFFWVSTPLITASDTEGAGEMFRVSTLDLENLPRNDQGRVDFDKDF
FGKESFLTVSGQLNGETYACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFADLED
NARLAEAMLKYVFNAVLEERADDMKFFAERVDKDAIARLERFVSTDFAQVDYTDAVAILE
RCGKTFENPVFWGVDLSSEHERYLAEEHFKAPVVVKNYPKEIKAFYMRLNEDGKTVAAMD
VLAPGIGEIIGGSQREERLDVLDARMAEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERL
IAYVTGVQNVRDVIPFPRTPRNASF