Protein Info for GFF3673 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 368 transmembrane" amino acids 88 to 107 (20 residues), see Phobius details TIGR03723: tRNA threonylcarbamoyl adenosine modification protein TsaD" amino acids 3 to 331 (329 residues), 421.1 bits, see alignment E=2.6e-130 TIGR00329: metallohydrolase, glycoprotease/Kae1 family" amino acids 4 to 325 (322 residues), 351.9 bits, see alignment E=2.9e-109 PF00814: TsaD" amino acids 28 to 325 (298 residues), 318.4 bits, see alignment E=2.4e-99

Best Hits

Swiss-Prot: 78% identical to TSAD_ACIAC: tRNA N6-adenosine threonylcarbamoyltransferase (tsaD) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K01409, O-sialoglycoprotein endopeptidase [EC: 3.4.24.57] (inferred from 78% identity to aaa:Acav_2996)

Predicted SEED Role

"TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (368 amino acids)

>GFF3673 TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA (Hydrogenophaga sp. GW460-11-11-14-LB1)
MRILGIESSCDETGVALVDASGSATPRLLGHALHSQIEMHQAYGGVVPELASRDHIRRVL
PLTDAVLAQAGLPLSAVDAVAYTRGPGLAGALLVGSGVACALGFALGKPVIGVHHLEGHL
LSPFLSADPPEFPFVALLVSGGHSQLMRVDGVGRYELLGETIDDAAGEAFDKSAKLLGLP
YPGGPALSRLAQQGDPQAYKFPRPLLHSGDLDFSFAGLKTAVLTQVKRLAHAHPDGPATL
HLADPLSAQQKADVAASVQAAIVEVLVKKSFMAIRRTGLRRLVVAGGVGANALLREQLGA
ACAQAGVRVHYPELHLCTDNGAMIALAGGMRAQAGLAGGDAAGAGGYPFDVKPRWPLSDL
GLPATPAG