Protein Info for GFF3665 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 822 PF07992: Pyr_redox_2" amino acids 5 to 289 (285 residues), 188.8 bits, see alignment E=5.2e-59 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 6 to 798 (793 residues), 926.4 bits, see alignment E=8.6e-283 PF13738: Pyr_redox_3" amino acids 72 to 283 (212 residues), 33.3 bits, see alignment E=1.1e-11 PF00070: Pyr_redox" amino acids 151 to 233 (83 residues), 65.8 bits, see alignment E=1.4e-21 PF18267: Rubredoxin_C" amino acids 324 to 390 (67 residues), 64.3 bits, see alignment E=2.8e-21 PF04324: Fer2_BFD" amino acids 428 to 475 (48 residues), 48.3 bits, see alignment 3.8e-16 amino acids 492 to 542 (51 residues), 48.6 bits, see alignment 2.9e-16 PF03460: NIR_SIR_ferr" amino acids 567 to 628 (62 residues), 51.9 bits, see alignment 1.8e-17 PF01077: NIR_SIR" amino acids 637 to 775 (139 residues), 103 bits, see alignment E=3.9e-33

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 86% identity to aaa:Acav_3047)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (822 amino acids)

>GFF3665 Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKKSKLVMVGNGMAGVRTLEELLKIAPDLYDITVFGAEPHPNYNRILLSPVLAGEQTLDE
IVLNDWAWYQDNGITLHAGKTVTEVDRIKRIVRASDGHGGVIEAPYDRLIMATGSNPFML
PIPGKELQGVLAYRDIADTQAMIDAATQYQHAIVIGGGLLGLEAANGLMKRGMTVSVVHV
APWLMERQLDDVAGKLLQQSLEQRGMRFLIGAQTQELVGNEAGRVKAIRFKDGTELPADL
VVMAVGIRPNTALAESMNLHVNKGIVVSDTLQTVTDPRIYAVGECAAHRGIAYGLVAPLF
EQGKVLATHLAEFGIGRYVGSLTSTKLKVTGIDLFSAGNFMGGEHTEEIVMSDPSGGVYK
KLVIEGDKLVGACLYGDTVDGSWYFKLLRDGRSVADIRDRLMFGESNIGDVGHQGHSKAA
AMADSDEVCGCNGVTKGSICKTIKDKGLFTLEEVRKHTKASASCGSCTGLVEQILMFTAG
GDYSATPKKKAMCSCTEHSHQEVRDAIFANKYLTIAEVQNGMGWKTPNGCSSCRPALNYY
LISSWPKEAKDDPQSRFINERSHANIQKDGTYSVIPRMWGGETTADELRRIADAVDKYKI
PTVKVTGGQRIDLLGVKKEDLVNVWNDIGMPSGHAYAKALRTVKTCVGSEWCRMGTQDST
QMGKDLERAHWRMYAPHKVKYAVSGCPRNCAEAGIKDVGIIGVDSGWEMYIAGNGGIKTE
VAHFFVKLKTAEEVLEYTGAFMQLYRKEGWYLERTVHYVSRVGLDYVKKRILEDAEGRKA
LNEELLFALDGEPDPWFEHQKAAVDTRQFIPIKPVSLEGSAA