Protein Info for GFF3663 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Assimilatory nitrate reductase large subunit (EC:1.7.99.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 926 PF04879: Molybdop_Fe4S4" amino acids 5 to 52 (48 residues), 62.9 bits, see alignment 4.5e-21 PF00384: Molybdopterin" amino acids 64 to 492 (429 residues), 231.2 bits, see alignment E=3.8e-72 PF01568: Molydop_binding" amino acids 591 to 709 (119 residues), 93.5 bits, see alignment E=1.7e-30 PF04324: Fer2_BFD" amino acids 866 to 916 (51 residues), 27.8 bits, see alignment 5.2e-10

Best Hits

KEGG orthology group: K00372, nitrate reductase catalytic subunit [EC: 1.7.99.4] (inferred from 75% identity to vap:Vapar_3530)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (926 amino acids)

>GFF3663 Assimilatory nitrate reductase large subunit (EC:1.7.99.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNPITTRSTCPYCGVGCGVLIESVGSQITGVRGDPQHPANLGKLCTKGQTLHLTASPAIA
QQTRLLHPMQRAQRGAPALPLAWDAALDLATDRFASVVERHGPDAVGFYISGQLLTEDYY
AFNKLAKGLIGTNNIDTNSRLCMSSAVAGYKLTLGADAPPACYDDVNHAQCLFIVGSNAA
FAHPVLFRRIEEARKNNPAMKVIVADPRRTDTAGLADLYLPLLPGSDVMLFHGLLHIMLW
EGWIDSGYIAAHTSGFDALKALVREHTPERVAQACGLRREDLFTAAKWFANAPATLSLYC
QGLNQSSSGTAKNATLINLHLATGQIGKPGAGPFSLTGQPNAMGGREVGGLANLLSAHRD
LANPEHRAEVAALWGVPSVPEKPGLSAVELFEAAADGQVKALWIACTNPAQSMPDQATVR
RALARAEFVVVQEAYATAATCAFADLLLPATTWGEKDGTVTNSERRISRVRAAVPAPGQA
RHDWRIAVDFAQRLEARWRPGQPTLFPYPDAESLWLEHRESTRGRDLDITGLSYAMLQQQ
GPQQWPLKAGESQGRERLYEDGVFATPDGKARFAALPFVPLAEPRESRYPFSLTTGRLRD
QWHGMTRTGTLGRLFGHVAEPSVQLHPQDMERRGLKDGDLVHVTSKRGSILVPAVASDEI
GLSQAFIAMHWGAEYLSGLSSTGEPLAGVNALTTSAYCPTSKQPELKHAAVKVLKAELPW
TLLAVGWLPEAKALEAREGLRALMAQFPFASCVPFGRERTGVLFRAAAHDAPEPALVERI
EALLGLQTADALRYVDKRLGQRRTARLVREGESTRLEAFVLAGDTRAEVWIKPLLQDELP
AAAYGRLLLMPGAKAPVGVVAKGRQVCTCFNVSEDAILEQLARCEGSDEQRLSALQAGLQ
CGTNCGSCVPELKRLVRASTPQRQAA