Protein Info for GFF363 in Methylophilus sp. DMC18

Annotation: Biosynthetic peptidoglycan transglycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 731 signal peptide" amino acids 1 to 38 (38 residues), see Phobius details TIGR02073: penicillin-binding protein 1C" amino acids 48 to 615 (568 residues), 599.3 bits, see alignment E=6.5e-184 PF00912: Transgly" amino acids 65 to 238 (174 residues), 150.7 bits, see alignment E=4.4e-48 PF00905: Transpeptidase" amino acids 318 to 586 (269 residues), 79.5 bits, see alignment E=4e-26 PF06832: BiPBP_C" amino acids 642 to 715 (74 residues), 35 bits, see alignment E=1.6e-12

Best Hits

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (731 amino acids)

>GFF363 Biosynthetic peptidoglycan transglycosylase (Methylophilus sp. DMC18)
MRGFFLPLWLSLRSRPWLCAVLALLAGICLLILWVATVKVPDFGQFKQHSQSSEAWLLDR
HGAPLQRLRLNHDYRRLDWVPLAQISPRLQQAMLVAEDKRFYQHAGVDPWALLSAIWDNL
HLPHRRGGSTLTMQLAKLWLQDTQPGFSNTSPWRFKLAQIRLALAIDHDWQKNQILEAYL
NRVTFRGELVGIDAAARGLLAKGPAALDRLDAALLAALVRAPAASASRLARRACDVLTHT
HTQDARQDCERLWIRAALLPKRPFPMAGQDDAPHVARRLLTQAGQRDRSSLDASLQRFAR
QTLQTHLAQLASQHVTDGAVLVLDNTSGEVLAYVGSSGDLSGAAAVDGVAALRQPGSTLK
PFLYALAIDQGWLQAASVLDDSPLALTTPSGLYIPQDYDRHFHGAVSVRTALASSLNVPA
VRALTLVGVDRFLHTLQQLGLQDLKQDADFYGFGLALGGAEASLFQLTNAYRALANGGQW
QPVSFHTSTKPLSPSRQVLTPQTSFIIGDILSDPVARSMTFGLSSPLSTRGWAAVKTGTS
KGMRDNWAIGYSQRYTVGVWVGNFSGEPMWDVSGVTGAAPVWRDIMEYLHRDSASTPPVP
PAGVVAQTVHYVPAIEAPRQEWVLGNAPLPAITTLRLAPLQAQLIAPPAGAVIAPDPDIP
DRKQALMVQAAWSGQGCLYLDQHLINQCRLPQLTIPLPPPGKHVLSLRDKHGQLLDQHSF
EVRAIALKAPA