Protein Info for GFF3591 in Sphingobium sp. HT1-2

Annotation: Glutamyl-tRNA synthetase (EC 6.1.1.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 76% identical to SYE2_SPHWW: Glutamate--tRNA ligase 2 (gltX2) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 90% identity to sjp:SJA_C1-08940)

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17)" in subsystem Heme and Siroheme Biosynthesis or tRNA aminoacylation, Glu and Gln (EC 6.1.1.17)

MetaCyc Pathways

adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
tRNA charging (19/21 steps found)
superpathway of heme b biosynthesis from glutamate (7/10 steps found)
tetrapyrrole biosynthesis I (from glutamate) (4/6 steps found)
adenosylcobalamin biosynthesis I (anaerobic) (25/36 steps found)
(2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.17

Use Curated BLAST to search for 6.1.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (490 amino acids)

>GFF3591 Glutamyl-tRNA synthetase (EC 6.1.1.17) (Sphingobium sp. HT1-2)
MDQAGRMSANMTASATGLNKQVVTRFAPSPTGFLHIGGARTALFNWLFARHHGGKFLLRI
EDTDRARSTEEAVAAIFDGLEWLNLGGDEPAVFQFERTPRHAEVANQLLANGHAYRCYAT
QEELAELREQQRAAKQPMRYDGRWRDRSPEEAPEGAPFVIRIKAPRTGETVIEDAVQGRV
VVQNAELDDMILLRSDGTPTYMLAVVVDDHDMGVTHVIRGDDHLNNAFRQLAIIKAMGWE
EPVYGHIPLIHGSDGAKLSKRHGALGVDAYRDEMGMLPEAVLNYLLRLGWGHGDEEIISI
ERAIELFDIDGVGRSPSRFDIKKLENLNGHYLREADEARLAALVAPRVESRLDTQLNDAQ
RDILTQAMVSLKPRAKTLNEIAEGAEFLFKSCPLDFDEKASALLDEPARELLKKTADALQ
PVQSWTVEAIDEVIRRVAEDAGLGLGKVAQPLRAALTGRTVSPGIFDVLFLLGKEESLKR
LTAAGHVVAG