Protein Info for PS417_18320 in Pseudomonas simiae WCS417

Annotation: phenylalanyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 792 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 791 (791 residues), 854.1 bits, see alignment E=5.6e-261 PF01588: tRNA_bind" amino acids 45 to 144 (100 residues), 80.3 bits, see alignment E=2.2e-26 PF03483: B3_4" amino acids 211 to 384 (174 residues), 246.4 bits, see alignment E=3.3e-77 PF03484: B5" amino acids 405 to 471 (67 residues), 73.3 bits, see alignment E=3.8e-24 PF17759: tRNA_synthFbeta" amino acids 474 to 682 (209 residues), 215.8 bits, see alignment E=1.3e-67 PF03147: FDX-ACB" amino acids 698 to 791 (94 residues), 104.3 bits, see alignment E=9.1e-34

Best Hits

Swiss-Prot: 92% identical to SYFB_PSEPF: Phenylalanine--tRNA ligase beta subunit (pheT) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 99% identity to pfs:PFLU4143)

MetaCyc: 52% identical to phenylalanine--tRNA ligase subunit beta (Escherichia coli K-12 substr. MG1655)
Phenylalanine--tRNA ligase. [EC: 6.1.1.20]; 6.1.1.20 [EC: 6.1.1.20]; 3.1.1.- [EC: 6.1.1.20]

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UC77 at UniProt or InterPro

Protein Sequence (792 amino acids)

>PS417_18320 phenylalanyl-tRNA synthetase (Pseudomonas simiae WCS417)
MKFSEQWLRGWVSPQVDRDELVARLSMAGLEVDSVTPAAGVFSGVVVGEVLSTEQHPDAD
KLRVCQVSNGAETFQVVCGAPNVRPGLKIPFAMIGAELPGDFKIKKAKLRGVESNGMLCS
QAELQVGEGNDGLMELPADAPVGQDIRVYLELEDASIEVDLTPNRGDCLSLAGLAREVGA
LYNAPVTRPVVAAVPAVHDEVRSIEVLAPNACPRYLGRVIRNVDLSKPTPLWMVERLRRA
DVRSIDAAVDITNYVMLELGQPLHAFDLAEINGGIRVRLAEEGEKLVLLDGQEVSLRADT
LVIADHSRALAIAGVMGGEHSGVSATTRDIFLESAFFDQIAIAGKARSYGLHTDASHRYE
RGVDWKLAREAMERATGLLLEITGGEAGPITETVNEQYLPSVAPITLRAKSVEQMLGLVI
EPAEIEQLLSALGLGISAGGEGQWTVEVPSHRFDISLEVDLIEELARLYGYNRLPVRYPQ
ARLAPQPKAEARAHLPELRRLLVARGYQEAVTYSFIDPKQFELFNPGVEPLLLANPISND
MAAMRSSLWPGLVKALSHNLNRQQDRVRMFESGLRFVGQLDGLKQEPMLAGVVCGSRLPE
GWAQGRDAVDFFDVKADVEAVLGFAGALDAFTFVPGSHPALHPGQTARIEREGRLVGFVG
AIHPELSKTLGLDRPVFVFELVLAEVASGKMPKFSELSRFPEVRRDLALIADREVAATAV
LEVIRENAGEWLTDLRLFDVYQGKGIDPHRKSLAVGLTWQHPSRTLNDDEVNTTTQNILT
SLEQRLNATLRK