Protein Info for GFF355 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: ATP-dependent DNA helicase RecG (EC 3.6.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 693 PF17191: RecG_wedge" amino acids 15 to 171 (157 residues), 74.8 bits, see alignment E=2.2e-24 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 28 to 664 (637 residues), 834.2 bits, see alignment E=4e-255 PF01336: tRNA_anti-codon" amino acids 63 to 134 (72 residues), 39.7 bits, see alignment E=1.4e-13 PF04851: ResIII" amino acids 268 to 430 (163 residues), 49 bits, see alignment E=2.4e-16 PF00270: DEAD" amino acids 270 to 436 (167 residues), 93.7 bits, see alignment E=3.7e-30 PF07517: SecA_DEAD" amino acids 297 to 363 (67 residues), 28.8 bits, see alignment E=2.7e-10 PF00271: Helicase_C" amino acids 479 to 587 (109 residues), 67.8 bits, see alignment E=3.4e-22 PF19833: RecG_dom3_C" amino acids 617 to 676 (60 residues), 41.5 bits, see alignment 4e-14

Best Hits

Swiss-Prot: 95% identical to RECG_ECO57: ATP-dependent DNA helicase RecG (recG) from Escherichia coli O157:H7

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 95% identity to cro:ROD_41641)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (693 amino acids)

>GFF355 ATP-dependent DNA helicase RecG (EC 3.6.1.-) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSGRLLDAVPLNSLTGVGAAQSSKLAKIGLHTVQDLLLHLPLRYEDRTHLYPIGELLPGI
YATVEGEVLNCNITFGGRRMMTCQISDGSGILTMRFFNFNAAMKNSLATGRRVLAYGEAK
RGKYGAEMIHPEYRLQGDLSTPELQETLTPVYPTTEGVKQATLRKLTDQALELLDTCAIA
ELLPPELAQGMMSLPEALRTLHRPPPTLQLADLETGKHPAQRRLILEELLAHNLSMLALR
AGAQRYHAQPLSTNNILKDKLLASLPFKPTSAQARVVAEIERDMALDVPMMRLVQGDVGS
GKTLVAALAALRAIAHGKQVALMAPTELLAEQHANNFRNWFEPLGVEVGWLAGKQKGKAR
QAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQHRFGVHQRLALWEKGQQQGFH
PHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRHEIIDRVRNACT
TEGRQAYWVCTLIEESDLLEAQAAEATWEELKLALPELNIGLVHGRMKPAEKQAVMQAFK
QGELHLLVATTVIEVGVDVPNASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKS
PLSKTAQKRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPE
VQRIARHIHERYPQQAQALIERWMPETERYSNA