Protein Info for GFF3549 in Pseudomonas sp. DMC3

Annotation: Phosphate transport system permease protein PstA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 transmembrane" amino acids 32 to 57 (26 residues), see Phobius details amino acids 88 to 113 (26 residues), see Phobius details amino acids 127 to 150 (24 residues), see Phobius details amino acids 157 to 177 (21 residues), see Phobius details amino acids 201 to 223 (23 residues), see Phobius details amino acids 276 to 298 (23 residues), see Phobius details TIGR00974: phosphate ABC transporter, permease protein PstA" amino acids 33 to 300 (268 residues), 263.8 bits, see alignment E=7.4e-83 PF00528: BPD_transp_1" amino acids 105 to 298 (194 residues), 53.9 bits, see alignment E=9.5e-19

Best Hits

Swiss-Prot: 39% identical to PSTA_ECOLI: Phosphate transport system permease protein PstA (pstA) from Escherichia coli (strain K12)

KEGG orthology group: K02038, phosphate transport system permease protein (inferred from 94% identity to pfs:PFLU3316)

MetaCyc: 39% identical to phosphate ABC transporter membrane subunit PstA (Escherichia coli)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate transport system permease protein PstA (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (303 amino acids)

>GFF3549 Phosphate transport system permease protein PstA (Pseudomonas sp. DMC3)
MTDLTAATDLTAPTGAMPSLQRRFEGRALRSLILTTLVWAGALLASVPLISVLYMLITRG
GARLNVEVFTELPPTGFEMGGGFGNAMAGTFVMVGIAAAIAVPVGIMAAIFLAELGPDSK
LGNAARFAAKMLTGLPSILAGVFAYALVVMTTGTYSAPAGGVALAVLMLPIVVLTAEESM
RMVPKIMKDAAYGMGCTRSQVIWKIILPTGMPAILTGVMLAVARAAGETAPLLFTALFSN
YWIYHQGNLDVMNPTASLAVLIYNFSGMPFDNQLELAWAASLVLVMIVLFVNIISRIFGK
PKY