Protein Info for GFF3533 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Thymidine phosphorylase (EC 2.4.2.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 703 PF07793: DUF1631" amino acids 3 to 255 (253 residues), 101.8 bits, see alignment E=2e-33 amino acids 281 to 688 (408 residues), 207.7 bits, see alignment E=1.8e-65

Best Hits

Predicted SEED Role

"Thymidine phosphorylase (EC 2.4.2.4)" in subsystem Deoxyribose and Deoxynucleoside Catabolism (EC 2.4.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.4

Use Curated BLAST to search for 2.4.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (703 amino acids)

>GFF3533 Thymidine phosphorylase (EC 2.4.2.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MDASNTQAFDACLREALAQTREWVPRWLGKLHSTLKDRELSAGQLSEKQGMLQARTTLES
HRELIATRFLAAFAESMENALPLTGTGSRRSPTVSFDELELMGDDQVQETVELARVQQVV
KMAVDEELVTLTSRLNGAQGLTVVNPEANPLRVEVVVAALMTALKGLHVEAAVRTRWLQV
GAITLGDELRNLYADLSRQLDRAGVKPAGYVVTQTATSRKVAAPSSQRDEAVSVQEVLGN
DNLLTLDHLHQLLVGNLDLNEKSNSKTAVSDNVMGRTLASEVVGLMLKRIAADERLLAPV
RTMVQDMKPALLQLARSEPRFFADKQNPARRLLDAITERSLAFTSEMDPGYNPFSNQVRG
IVRTLLKPGEDLAERFPSLLAELHRSQVEAVAPAQVQARGLAVQTLVRVEQRNLLAERVA
AEIRARNDFEKAPGVVRRFLTGPWSQVVAQARLEVSESSTASPVDSPARRYSDILPDLLW
SSQLALASLNRPRLVKVIPGVLRTLREGLDAIDYPREQTEAFFQALMGLHEAAYKTQRSS
HEPPAEEEQPSIQPEMDDEPWMQRTEARDSCLMDDVLLDSQTSTQPAFLETRPMQRQRDW
ADVKAEMAVQRTSDLPVGTWVDVLQDGQAMRWQITWASPHGTLFLFTGANGRSLSMTKRG
LERLLEQDRLRVVADHGVVDEALDEVARQAWLNSAMAQDKKGP