Protein Info for GFF3530 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: ATP-dependent RNA helicase RhlE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 608 PF00270: DEAD" amino acids 39 to 212 (174 residues), 158.2 bits, see alignment E=2.4e-50 PF04851: ResIII" amino acids 41 to 207 (167 residues), 26.5 bits, see alignment E=8.6e-10 PF00271: Helicase_C" amino acids 248 to 356 (109 residues), 100.3 bits, see alignment E=1.2e-32

Best Hits

KEGG orthology group: K11927, ATP-dependent RNA helicase RhlE [EC: 3.6.4.13] (inferred from 74% identity to vap:Vapar_3682)

Predicted SEED Role

"ATP-dependent RNA helicase RhlE" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (608 amino acids)

>GFF3530 ATP-dependent RNA helicase RhlE (Hydrogenophaga sp. GW460-11-11-14-LB1)
VADAPSALDGRLFYMTFDDLNLAPAIVQAVREHGYETPTPIQVEAIPAVLAGRDLLGGAQ
TGTGKTAAFTLPMLHRLANSERAKSRFGGVGIRALVLTPTRELAAQVEESVRVYGKHLSL
TSTVVFGGVGMNPQINAMKRGVDILVATPGRLLDLQQQGFLDLSTVQMLVLDEADRMLDM
GFIHDVKKILALVPKEKQSLLFSATFSDEIRDLASALLKDPLHIQVTPRNTTVQRITQVI
HPVGRGKKKALLVHIINQHNWSQVLVFTRTKFGANNVAEYLTKNGIHAMALHGNKSQGAR
TQALAGFKSGEIRALVATDIAARGIDIDELPHVVNYEIPNISEDYVHRIGRTGRAGNSGE
AVNLVSLDEEGFMMEIERFTKQQIPVQIFPEFMPEPGEKAEPIAMGRQTIWGGAGKPPSR
EVMAAAAKAARTEMLQRVREKKANEPQGRGNGGGGGQRQRPAGDGSQADGPRGEGRRGNG
GGQRANGNGGGGNGQRQGQRHGGGQQGQRPPREDRGFREDRGPREEREPGAERQPGPNAH
LGSLRIREPRPRAANPGGQPDPLRTSIDAMRTGNRGGKGKGGGGGGGQRGGNAGGPADPL
RTSYGRIR