Protein Info for GFF3529 in Variovorax sp. SCN45

Annotation: Nicotinamide-nucleotide adenylyltransferase, NadM family (EC 2.7.7.1) / ADP-ribose pyrophosphatase (EC 3.6.1.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00125: cytidyltransferase-like domain
  • amino acids 12 to 71 (60 residues), 56.3 bits, E=1.4e-19
• PF01467: CTP_transf_like
  • amino acids 13 to 74 (62 residues), 52.6 bits, E=5.8e-18
• PF00293: NUDIX
  • amino acids 217 to 338 (122 residues), 78.7 bits, E=4.3e-26

Best Hits

KEGG orthology group: K13522, bifunctional NMN adenylyltransferase/nudix hydrolase [EC: 2.7.7.1 3.6.1.-] (inferred from 93% identity to vpe:Varpa_5680)

Predicted SEED Role

"Nicotinamide-nucleotide adenylyltransferase, NadM family (EC 2.7.7.1) / ADP-ribose pyrophosphatase (EC 3.6.1.13)" in subsystem NAD and NADP cofactor biosynthesis global or Nudix proteins (nucleoside triphosphate hydrolases) or NAD regulation (EC 2.7.7.1, EC 3.6.1.13)

MetaCyc Pathways

• superpathway of NAD biosynthesis in eukaryotes (11/14 steps found)
• NAD biosynthesis from nicotinamide (2/2 steps found)
• NAD salvage pathway IV (from nicotinamide riboside) (1/2 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.1.13

Use Curated BLAST to search for 2.7.7.1 or 3.6.1.- or 3.6.1.13

Protein Sequence (359 amino acids)

>GFF3529 Nicotinamide-nucleotide adenylyltransferase, NadM family (EC 2.7.7.1) / ADP-ribose pyrophosphatase (EC 3.6.1.13) (Variovorax sp. SCN45)
MNTQNTRKYQYAIVIGRFQPVHFGHQRLIEEASRAADRVIVVVGSDRQPRSVKNPFTFDE
RERMVRACLRSSDQMRVSVVGVGDSPYNDQIWIASIQSAVERLIAQDGLAPAKARVALVG
HLKDASSYYLKMFPHWEFVGQNSVESLNATDVRSLYFDPENRGQLARADLPDGTAAFLDQ
FTRTADYAELCEERAFLVDYRDKYRYAGSDFPPIYTTVDAVVVEAGHILLVQRKFHPGKG
TWALPGGFVGQQERLQEAAIRELKEETRLKVPVPVLNGSMKASHVFDAPERSQRGRTITH
GFFFELAHNQWTGLSDVRADDDAAEVRWVPIAEFLDMQERIYEDHFFIANHFLGGLGKN