Protein Info for GFF3520 in Variovorax sp. SCN45

Annotation: Osmosensitive K+ channel histidine kinase KdpD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 917 transmembrane" amino acids 442 to 464 (23 residues), see Phobius details amino acids 472 to 488 (17 residues), see Phobius details amino acids 501 to 525 (25 residues), see Phobius details PF02702: KdpD" amino acids 23 to 232 (210 residues), 320.9 bits, see alignment E=8e-100 PF13493: DUF4118" amino acids 426 to 526 (101 residues), 101.3 bits, see alignment E=6.4e-33 PF13492: GAF_3" amino acids 593 to 666 (74 residues), 44.9 bits, see alignment E=3.9e-15 PF00512: HisKA" amino acids 686 to 749 (64 residues), 53.1 bits, see alignment 7e-18 PF02518: HATPase_c" amino acids 798 to 907 (110 residues), 82.4 bits, see alignment E=7.9e-27

Best Hits

KEGG orthology group: K07646, two-component system, OmpR family, sensor histidine kinase KdpD [EC: 2.7.13.3] (inferred from 93% identity to vpe:Varpa_5690)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (917 amino acids)

>GFF3520 Osmosensitive K+ channel histidine kinase KdpD (Variovorax sp. SCN45)
MLDDRPDPDALLAQLRSDEARAQRGKLRIYFGASAGVGKTWAMLSAAQRERAAGRDVLIG
VVETHGRSETAALLAGLEALPLRELAYRGRTLAEFDLDAALARKPAVLLVDELAHTNAPG
SRHAKRWQDVQELLAAGIEVWSALNVQHLESLNGTVGAITGVRVHETVPDTVLDEADEVV
LVDVTPDELTARLAAGKVYLPQQAERAAQNFFRKGNLIALREIALRRTAEHVEDDVRGWR
VEQSGPGAGGNGKGRGLQAWNTSGAILACVGPHEGAAQTVRTAARLAGQLNVRWHAVYVE
TPRLQRLAAAERDRILAVLKLAEELGAATAVLTGSDVAEQLAEQARRLNCATLVIGRPEP
ATGWRRWWPAAASPPLSRALAQRAPALDIMEVAPADSSRRLSRAPLEGARIDSDDHEKAP
TYWPGYAWATATSVALTLICTPLAGVLELSNIVMLFLLGVVGVAMRFGRGPSALAALLNV
AAFDYFFVPPRMSFAVSDVQYVLTFAIMLGVGLLVGQLTAGLRFAAGVSTSRERRARSLF
ELTRELSAALESSQVVALGTAAVQGHFGGHALVLVTDAADQLVLPAHAPEGFDPQVADWA
FRHGQPAGLATATLAAQPWHYVPLRAPMRVRGVLALSPAQPRWLLIPEQAQQLDTLARQI
AIALERVHYVEVAQQAVVEMESERLRNALLGAISHDVRTPLTALIALAESLQTLPPEEHD
NAARAIVAQAHELHALVNNLLDMARLESGIAGGAVNLRRDWQSVEEVVGSAIRAARTSLG
NTVVRTALDAELPLVEFDAVLIERVLVNLLENATKYGAPPIEVGARAEPGTLVLTVRDHG
PGLPAALLGHEQKLFDKFTRGEVESATPGVGLGLAICKAVVSAHGGEITAGNARDGGAEF
TVTLPRRDPPEPAEAQL