Protein Info for GFF3501 in Variovorax sp. SCN45

Annotation: Putative short-chain dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 254 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00106: adh_short" amino acids 7 to 201 (195 residues), 105.2 bits, see alignment E=6.3e-34 PF08659: KR" amino acids 8 to 171 (164 residues), 30.4 bits, see alignment E=7.5e-11 PF01370: Epimerase" amino acids 8 to 54 (47 residues), 22.8 bits, see alignment 1.2e-08 PF13561: adh_short_C2" amino acids 11 to 203 (193 residues), 71.6 bits, see alignment E=1.5e-23

Best Hits

KEGG orthology group: None (inferred from 88% identity to vpe:Varpa_5706)

Predicted SEED Role

"D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30)" in subsystem Polyhydroxybutyrate metabolism (EC 1.1.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.30

Use Curated BLAST to search for 1.1.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (254 amino acids)

>GFF3501 Putative short-chain dehydrogenase (Variovorax sp. SCN45)
MSISHLTLITGASRGLGRAMAEQLLQAGHTVLGISRKQSPELAEAAKAAGAELVQWEQDL
SDPVAASERVSAWLKTVDAQRFDSVTLINNAGTVGNPAPLSRAVEAELSLALRIGLEAPM
LITAAFLGATREWRGTRKVLNISSGLGRNAMGSQAPYCAAKAGMDHFSRAVALEEANAPN
GARIVSLAPGVIDTDMQVQLRGASADKFPDRTRFEKMKNEGMLDSPAAAATKVLGYLARE
DFGAKPVADVRDPA