Protein Info for GFF3495 in Pseudomonas sp. DMC3

Annotation: 1,4-alpha-glucan branching enzyme GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 743 PF22019: GlgB_N" amino acids 20 to 109 (90 residues), 57.3 bits, see alignment E=2.7e-19 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 119 to 740 (622 residues), 924.2 bits, see alignment E=1.9e-282 PF02922: CBM_48" amino acids 135 to 217 (83 residues), 80.4 bits, see alignment E=2e-26 PF00128: Alpha-amylase" amino acids 291 to 362 (72 residues), 34.4 bits, see alignment E=3.1e-12 PF02806: Alpha-amylase_C" amino acids 644 to 741 (98 residues), 86.8 bits, see alignment E=2e-28

Best Hits

Swiss-Prot: 94% identical to GLGB_PSEPF: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 94% identity to pfo:Pfl01_2536)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (743 amino acids)

>GFF3495 1,4-alpha-glucan branching enzyme GlgB (Pseudomonas sp. DMC3)
MSFSNKEQGVVKEALLPSARDIDALVRAEHQDPFSILGPHGDGAGGQFIRAYLPGALSVA
VLDKDSGEELGPLEMTETPGLFVGHFDQARPYLFRTRWAGGEQVAEDPYSFGQLLGEMDL
YLFAEGNHRDLSACLGAQLKTVDGVDGVRFAVWAPNARRVSVVGDFNVWDGRRHPMRLRH
PSGVWELFIPRLQAGELYKYEILGAHGILPLKADPMALATSLPPDTASKVASPLQIDWQD
HDWMNSRQDRQQRSKPLSIYELHAGSWQCELDDLGEVARQYTWPELAERLIPYVKELGFT
HIELMPIMEHPFGGSWGYQLLSQFAPSARYGTPDQFAAFVNACHQADIGVILDWVPAHFP
TDTHGLAQFDGTALYEYGNPLEGFHQDWDTLIYNLGRTEVHGYMLASALHWLKHFHIDGL
RVDAVASMLYRDYSRKAGEWVPNRHGGRENLEAIDFLRHLNDVVELEAPGALVIAEESTA
WPGVSQSTQQGGLGFSYKWNMGWMHDSLHYIQQDPVYRAHHHNELSFGLVYAWSERFILP
ISHDEVVHGKHSLIDKMPGDRWQKFANLRAYLSFMWTHPGKKLLFMGCEFGQWREWNHDQ
QLDWYLLQYSEHKGVQKLVGDLNRLYREEPALHDQDDAPQGFQWLIGDDAINSVYAWLRW
SKDGSPVLVVANFTPVPRQSYRVGVPFAGRWTELFNSDADIYAGSNYGNGGGALTDEVPS
HGQSLSLELNLPPLAVLILKPQG