Protein Info for GFF3486 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: DNA topoisomerase III (EC 5.99.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 649 TIGR01056: DNA topoisomerase III" amino acids 1 to 645 (645 residues), 998.3 bits, see alignment E=1.1e-304 PF01751: Toprim" amino acids 3 to 135 (133 residues), 55.6 bits, see alignment E=5.1e-19 PF01131: Topoisom_bac" amino acids 159 to 593 (435 residues), 370.2 bits, see alignment E=1.5e-114

Best Hits

Swiss-Prot: 100% identical to TOP3_SALTY: DNA topoisomerase 3 (topB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 95% identity to eco:b1763)

MetaCyc: 95% identical to DNA topoisomerase III (Escherichia coli K-12 substr. MG1655)
5.99.1.2-RXN [EC: 5.6.2.1]

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.6.2.1 or 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (649 amino acids)

>GFF3486 DNA topoisomerase III (EC 5.99.1.2) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MRLFIAEKPSLGRAIADVLPKPHRKGDGFIECGNGQVVTWCIGHLLEQAQPDAYDSRYAR
WNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHQAGEIIHAGDPDREGQLLVDEVLDYLQ
LPAEKRQQVRRCLINDLNPQAVERAIDRLRANSDFVPLCVSALARARADWLYGINMTRAY
TILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVAKDFFEVKAHIVTPADERFTAIW
QPSEACEPYQDEEGRLLHRPLAEHVVNRINGQPALVTSYNDKRESESAPLPFSLSTLQIE
AAKRFGLSAQNVLDICQKLYETHKLITYPRSDCRYLPEEHFAGRQAVMNAISVHAPDLLP
QPVVNPDTRNRCWDDKKVDAHHAIIPTARSSSVHLTENEAKVYTLIARQYLMQFCPDAVF
RKCVIELEIAKGKFVAKARFLAEAGWRTLLGSKERDEENDGTPLPVVAKGDELLCEKGEV
VERQTQPPRHFTDATLLSAMTGIARFVQDKDLKKILRATDGLGTEATRAGIIELLFKRSF
LTKKGRYIHSTDAGKALIHSLPEMAARPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLY
QLIEQAKRTPVKRFRGIVAPGGGDKKKSAPRKRAGKKSPPAAETGRQTE