Protein Info for GFF3483 in Pseudomonas sp. DMC3

Annotation: 1,4-alpha-glucan branching enzyme GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 transmembrane" amino acids 401 to 418 (18 residues), see Phobius details TIGR02402: malto-oligosyltrehalose trehalohydrolase" amino acids 21 to 551 (531 residues), 677.2 bits, see alignment E=6.9e-208 PF00128: Alpha-amylase" amino acids 125 to 212 (88 residues), 42.7 bits, see alignment E=4.8e-15 PF11941: DUF3459" amino acids 496 to 584 (89 residues), 63.3 bits, see alignment E=2.3e-21

Best Hits

KEGG orthology group: K01236, maltooligosyltrehalose trehalohydrolase [EC: 3.2.1.141] (inferred from 88% identity to pfo:Pfl01_2548)

Predicted SEED Role

"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (600 amino acids)

>GFF3483 1,4-alpha-glucan branching enzyme GlgB (Pseudomonas sp. DMC3)
MPSRTQETWPHGAIMLDAEHTQFALWAPDAFYVSVELEDGKSLAMLPQGDGWFVIKANCP
AGSRYRYCIDGELEVPDPASRAQDGDLDRHSVVVDPHAYQWRHTTWNGRPWSEAVIYELH
VGALGGFAEVEQHLARLVALGITAIELMPLAQFPGDRNWGYDGVLPYAPQASYGTPEQLK
HLIDTAHGHGLAVILDVVYNHFGPDGNYLHRYAKGFFREDKHTPWGAAIDFRRPEVREFF
IENALMWLLEYRFDGLRLDAVHAIEDPDFLSDLARRIRQQIDPTRHVWLTVENELNQSSL
LENDYDAQWNDDGHNALHVLLTGETDAYYADYAAQPTEQLVRCLSQGFVFQGHITRHGEP
RGEPSEHLPSTAFVLFLQNHDQIGNRAFGERLHQLADPRAVQAATVLLLLSPMIPLMFMG
DEFAAEQPFLFFTSHHGELAELVREGRRNEFAAFSAFTDPHKREQIPDPNAEETFRASQP
RLVGSGTPLQQETQELYRQLLKLRHQYIIPNLSGTQALGAQMLGHGAVSARWRLGNGSEL
RIDLNLSDTPVVNPAQTDTEWLFQQPAAIELSDPNQLPAYTALVSLTAAPVMHPLDGERL