Protein Info for PS417_17830 in Pseudomonas simiae WCS417

Annotation: 4-hydroxyphenylacetate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 221 TIGR02305: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, N-terminal subunit" amino acids 11 to 215 (205 residues), 286.2 bits, see alignment E=6.3e-90 PF01557: FAA_hydrolase" amino acids 13 to 215 (203 residues), 181.1 bits, see alignment E=1.3e-57

Best Hits

KEGG orthology group: K05921, 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase [EC: 4.1.1.68 5.3.3.-] (inferred from 76% identity to pen:PSEEN3092)

Predicted SEED Role

"5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic amino acid degradation (EC 4.1.1.68, EC 5.3.3.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.68, 5.3.3.-

Use Curated BLAST to search for 4.1.1.68 or 5.3.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UN95 at UniProt or InterPro

Protein Sequence (221 amino acids)

>PS417_17830 4-hydroxyphenylacetate isomerase (Pseudomonas simiae WCS417)
MSRTLHDVASGTLFGVALNYQGLLDQHLAAFQQAPYQKPPTKPVLFIKTPNTRNQHDAVV
LHPQGERLQPGPALGVVIGLRASRVSLENAMAHVAGYVVVNEFSLPEDSYYRPAVKAKCR
DGFCALGPHLIPRDQVANPNQLSLRLWVNGELRQENSTAHWVRDIAQLIAEISEFMTLHP
GDVLITGTPEGRVDVLPGDKVEVEISGVGRLVNHIQAETPA