Protein Info for PS417_01765 in Pseudomonas simiae WCS417

Annotation: N-formylglutamate amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02017: N-formylglutamate deformylase
  • amino acids 4 to 262 (259 residues), 394.7 bits, E=1e-122
• PF05013: FGase
  • amino acids 13 to 230 (218 residues), 249.5 bits, E=2.2e-78

Best Hits

KEGG orthology group: K01479, formiminoglutamase [EC: 3.5.3.8] (inferred from 94% identity to pfs:PFLU0370)

MetaCyc: 77% identical to N-formylglutamate amidohydrolase (Pseudomonas putida)
N-formylglutamate deformylase. [EC: 3.5.1.68]

Predicted SEED Role

"N-formylglutamate deformylase (EC 3.5.1.68)" in subsystem Histidine Degradation (EC 3.5.1.68)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation I (4/4 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.68 or 3.5.3.8

Sequence Analysis Tools

See A0A1N7UEP0 at UniProt or InterPro

Protein Sequence (266 amino acids)

>PS417_01765 N-formylglutamate amidohydrolase (Pseudomonas simiae WCS417)
MDKVLNFKQGRVPLLISMPHAGLRLTPAVEAGLIPEAQSLPDTDWHIPTLYDFAEELGAS
TLAAEYSRFVIDLNRPSDDKPLYAGATTGLYPATLFDGVPLFREGLEPSEAERASYLQKV
WGPYHRTLQEELARLKAEFGYALLFDAHSIRSVIPHLFDGKLPDFNLGTFNGAACDPELA
NQLEAICAAHPQYTHVLNGRFKGGHITRHYGDPAQDIHAVQLELCQSAYMEEFEPFRYRP
DLAEPTRVVLKQLLQELLAWGQKRYG