Protein Info for GFF3444 in Sphingobium sp. HT1-2

Annotation: UDP-N-acetyl-D-mannosamine dehydrogenase (EC 1.1.1.336)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03721: UDPG_MGDP_dh_N
  • amino acids 7 to 189 (183 residues), 171.6 bits, E=3.5e-54
• PF03446: NAD_binding_2
  • amino acids 8 to 138 (131 residues), 25 bits, E=4.5e-09
• TIGR03026: nucleotide sugar dehydrogenase
  • amino acids 8 to 411 (404 residues), 392.1 bits, E=1.3e-121
• PF00984: UDPG_MGDP_dh
  • amino acids 208 to 295 (88 residues), 99.1 bits, E=3.2e-32
• PF03720: UDPG_MGDP_dh_C
  • amino acids 318 to 414 (97 residues), 73.3 bits, E=4.7e-24

Best Hits

Swiss-Prot: 54% identical to EPSD_RALSO: NDP-N-acetyl-D-galactosaminuronic acid dehydrogenase (epsD) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K02472, UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase [EC: 1.1.1.-] (inferred from 90% identity to sjp:SJA_C1-22180)

MetaCyc: 55% identical to UDP-N-acetyl-D-mannosamine dehydrogenase (Escherichia coli K-12 substr. MG1655)
UDPMANNACADEHYDROG-RXN [EC: 1.1.1.336]

Predicted SEED Role

"UDP-glucose dehydrogenase (EC 1.1.1.22)" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance ) or Teichuronic acid biosynthesis (EC 1.1.1.22)

MetaCyc Pathways

• UDP-α-D-xylose biosynthesis (2/2 steps found)
• UDP-α-D-glucuronate biosynthesis (from UDP-glucose) (1/1 steps found)
• UDP-N-acetyl-α-D-mannosaminouronate biosynthesis (1/1 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• colanic acid building blocks biosynthesis (7/11 steps found)
• UDP-sugars interconversion (4/9 steps found)
• superpathway of enterobacterial common antigen biosynthesis (3/10 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Benzoate degradation via CoA ligation
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of type II polyketide products
• Biosynthesis of unsaturated fatty acids
• Bisphenol A degradation
• Butanoate metabolism
• C21-Steroid hormone metabolism
• Fructose and mannose metabolism
• Glycine, serine and threonine metabolism
• Insect hormone biosynthesis
• Linoleic acid metabolism
• Nucleotide sugars metabolism
• Pentose and glucuronate interconversions
• Polyketide sugar unit biosynthesis
• Retinol metabolism
• Starch and sucrose metabolism
• Tetrachloroethene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.-, 1.1.1.22

Use Curated BLAST to search for 1.1.1.- or 1.1.1.22 or 1.1.1.336

Protein Sequence (431 amino acids)

>GFF3444 UDP-N-acetyl-D-mannosamine dehydrogenase (EC 1.1.1.336) (Sphingobium sp. HT1-2)
MPVDSKQKVSVIGLGYIGLPTAALIARGGAQVVGVDVSAHVVETVNSGRVHIEEVDLDGL
VQGVVARGNLRASLSVEASDVFIIAVPTPVAEDRAPDISYVLQAARTVAPVLKNGDTVIL
ESTSPVGTTEAMRDLIAELRPDLKMPAPGVAGDIAIAYCPERVLPGRILVELIDNDRCIG
GITPRCARKALGFYRQFVRGACITTTARAAEMVKLVENSFRDVNIAFANELSVIADRMDI
DVWEVIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVHGDPENARIIRTAREVNDGKTDY
VVAKSSDMIDAFAGEDVACLGLAFKPNIDDFRESPAVKVASRLARRYGKRVKLVEPYAQD
LPMEFAGTGAELIDLDTALEQCGVFIILVDHDMFKSVPVDERAGKAVYDTRGIWPDQPRR
QPEPAAQRIAV