Protein Info for PS417_17580 in Pseudomonas simiae WCS417

Updated annotation (from data): citrulline utilization hydrolase
Rationale: Important on citrulline. This enzyme is distantly related to characterized arginine deiminases (see PF02274) and has conserved catalytic residues. It might be a citrullinase.
Original annotation: amidinotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: None (inferred from 82% identity to pen:PSEEN1860)

MetaCyc: 65% identical to citrullinase (Pseudomonas fluorescens)
Citrullinase. [EC: 3.5.1.20]

Predicted SEED Role

No annotation

MetaCyc Pathways

L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
L-citrulline degradation (3/3 steps found)
cyanate degradation (3/3 steps found)
urea degradation I (2/3 steps found)
superpathway of allantoin degradation in yeast (4/6 steps found)
cyanuric acid degradation II (3/5 steps found)
cyanuric acid degradation I (2/5 steps found)
uracil degradation III (2/5 steps found)
superpathway of atrazine degradation (3/8 steps found)
allantoin degradation IV (anaerobic) (2/9 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UE28 at UniProt or InterPro

Protein Sequence (308 amino acids)

>PS417_17580 citrulline utilization hydrolase (Pseudomonas simiae WCS417)
MQTTNTVLMIRPARFAFNPDTAINNRFQRQPLDPLGAQQKALEEFDGYVDTLRRHGVEVL
VVQDTPAPHTPDSIFPNNWWSSHADGSLVLYPMEGQNRRLERNKGVLQVLEQRFAINSTI
DLSHLEQQNIFLEGTGSMVLDRQHRISYACHSGRTHQDALRQFAERLDYQLCVFHAVDRH
HAPIYHSNVMMSVGRDLSVVCLQALPDASERQGLERSLRDTGKDILALDFDQLEGFAGNM
LEIHDRDGQPLLVMSASAWGALQPAQRQHVERHTRPVVVNIDNIERIGGGSARCMLAEVH
LPARATVQ