Protein Info for PS417_01745 in Pseudomonas simiae WCS417

Annotation: histidine ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 TIGR01225: histidine ammonia-lyase" amino acids 8 to 497 (490 residues), 542.9 bits, see alignment E=3.2e-167 PF00221: Lyase_aromatic" amino acids 14 to 471 (458 residues), 557.1 bits, see alignment E=1.5e-171

Best Hits

Swiss-Prot: 46% identical to HUTH_SYMTH: Histidine ammonia-lyase (hutH) from Symbiobacterium thermophilum (strain T / IAM 14863)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 95% identity to pfs:PFLU0366)

MetaCyc: 38% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.3

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TVH6 at UniProt or InterPro

Protein Sequence (502 amino acids)

>PS417_01745 histidine ammonia-lyase (Pseudomonas simiae WCS417)
MSQATKITIADAPLRWQDVAAVARHGAVLELSGQAWARIDNAQAIVQRIVTSGERAYGVN
TGLGALCNVSLKGEQLSQLSRNTLLSHACGVGPVLSDEQTRAIICAAIINYSHGKSGLHP
QVVHSLLALLNHGITPQVPSQGSVGYLTHMAHVGVALLGVGTVSYQGRIVPAQEALSAEG
LHPVVLGAKDGLCLVNGTPCMTGLSCLALADAHHLLQWADVIGAMSFEAQRGQIDAFDEE
IIALKPHPGMQQVGINLRALLDGSEVIASSQGIRTQDALSIRSIPQIHGAARDQVEHATR
QIETELNSATDNPLVLGTPDHYRVVSQANPHGQSVALAADMLAIAMAEIGAVAERRLDRL
INPHVSGLPAFLVSNPGVNSGMMIVQYVAASLCGQNRQLAQPAVLDNFVTSGLQEDHLSM
GTNAALKLHQVLANVTQILAIEYLLAAQAFEFLKEQRFGAGTDRAWRLLREVVPAYEQDR
WLAPDIAAAAKLLKDTDLPNFH