Protein Info for GFF3404 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Butyryl-CoA dehydrogenase (EC 1.3.99.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 76% identity to ppw:PputW619_3347)MetaCyc: 44% identical to L-cysteinyl-[NRPS] dehydrogenase (Streptomyces clavuligerus)
RXN-16875
Predicted SEED Role
"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)
MetaCyc Pathways
- 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
- pyruvate fermentation to butanol II (engineered) (4/6 steps found)
- pyruvate fermentation to hexanol (engineered) (7/11 steps found)
- holomycin biosynthesis (2/12 steps found)
KEGG Metabolic Maps
- Butanoate metabolism
- Fatty acid metabolism
- Propanoate metabolism
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.3.99.2, 1.3.99.3
Use Curated BLAST to search for 1.3.99.2 or 1.3.99.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (383 amino acids)
>GFF3404 Butyryl-CoA dehydrogenase (EC 1.3.99.2) (Hydrogenophaga sp. GW460-11-11-14-LB1) MLPRLHQHPWMDADIEGFREQVRRCVADKLAPKLDAWRRQGFIPRESWRSLGELGFLLPE MPEAYGGPGASIAYQLVVQDELSKQEIPANTGVHSIASHYILDYGTEAQKQRWLPRLVSG EMLAGIAMTEPGAGSDLKAIRTKARLDGDHYVVDGAKTFITNGFTANLLVVVVRTGEAGA KGVSLIVMETENLPGFSVGRRLEKLGQHASDTAELSFDGVRVPASQLLGGQEGEGFKQLM SQLTYERLLLAVNAAAVIERAVELTVDYTQQRKAFGQALADFQNTRFKLAECATLAHVVR TFVNDCIQRQLDGTLDNEAAYMAKWWCSEQQGKVTDECLQLFGGYGYMAEYPIARLYADS RVQRIYGGANEIMKDLIARKVLG