Protein Info for GFF340 in Variovorax sp. SCN45

Annotation: Cardiolipin synthase, bacterial type ClsA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 517 transmembrane" amino acids 48 to 67 (20 residues), see Phobius details amino acids 78 to 96 (19 residues), see Phobius details TIGR04265: cardiolipin synthase" amino acids 52 to 517 (466 residues), 390.9 bits, see alignment E=5.1e-121 PF13396: PLDc_N" amino acids 60 to 97 (38 residues), 31.2 bits, see alignment 2.5e-11 PF13091: PLDc_2" amino acids 171 to 280 (110 residues), 40.1 bits, see alignment E=4.7e-14 amino acids 361 to 486 (126 residues), 106.5 bits, see alignment E=1.4e-34 PF00614: PLDc" amino acids 254 to 278 (25 residues), 22.2 bits, see alignment (E = 1.7e-08) amino acids 436 to 459 (24 residues), 25.5 bits, see alignment (E = 1.5e-09)

Best Hits

KEGG orthology group: K06131, cardiolipin synthase [EC: 2.7.8.-] (inferred from 92% identity to vpe:Varpa_3262)

Predicted SEED Role

"Cardiolipin synthetase (EC 2.7.8.-)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.7.8.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.8.-

Use Curated BLAST to search for 2.7.8.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (517 amino acids)

>GFF340 Cardiolipin synthase, bacterial type ClsA (Variovorax sp. SCN45)
VADEATEQPPKAGSIALPAFFFGSAEPHRRLNPFFALILPELSHEWKTGLSLAWSGYIAV
LSVWIVMQKRAPVSTMSWILSLALLPFAGFVVYYFLGPQRLRKQRLKRLRSRAGASAQAD
VARLRDAAQNAPPALQQMARLGMATCGLPVSSALGVELLSGGARTFDAIFDAVRAARDHI
HLEYYIFEPDRIGTALRDLLVERAKEGVTVRLLIDALGSKRIGRKFMAPLHEAGVQVALF
HDTKIGRRLRPVTNYRTHRKIVVCDGRVGFTGGVNITDEEDKRTNPDAYHDVHLRIEGSA
VRWLQTTFLEDWTYATGEDPRGMDAGLDAMLPLLEAGSIPVQIVTSGPDNALEAIHRMHV
EAIHSATHRAWLTTPYFVPGEPALMALTSAALRGVDVRLLVPRRSDSAIVSAAARSYFDE
LIAAGVKVWEYRARMLHSKTLVVDDHCAMIGTANFDNRSFRLNFEVCAVVYGPALAGPLA
AQFETDLHSSGAVRANRRQSFWRRLGDAIARLFSPLL