Protein Info for GFF3399 in Variovorax sp. SCN45

Annotation: Benzoate--CoA ligase (EC 6.2.1.25)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02262: benzoate-CoA ligase family
  • amino acids 6 to 510 (505 residues), 793.4 bits, E=4.6e-243
• PF00501: AMP-binding
  • amino acids 20 to 377 (358 residues), 284.1 bits, E=1.6e-88
• PF13193: AMP-binding_C
  • amino acids 427 to 502 (76 residues), 80.2 bits, E=1.7e-26

Best Hits

KEGG orthology group: K04110, benzoate-CoA ligase [EC: 6.2.1.25] (inferred from 92% identity to vpe:Varpa_0102)

Predicted SEED Role

"Benzoate-CoA ligase (EC 6.2.1.25)" in subsystem Benzoate transport and degradation cluster (EC 6.2.1.25)

MetaCyc Pathways

• benzoate degradation II (aerobic and anaerobic) (1/1 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (6/17 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (6/27 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.25

Protein Sequence (515 amino acids)

>GFF3399 Benzoate--CoA ligase (EC 6.2.1.25) (Variovorax sp. SCN45)
MTPPARFNFAEHLFALNRGRAARTAYIDDRGTLSYGQLEERARRLAAALKAAGIRREERV
LLLMLDGNDWPVSFLGCLYAGIVPVAVNTLLTPDDYAYMLDHSRAQAVLVSGALLPTLQE
AMARGGHEVQAMFVSQPAGPLPDGAREFDAAIAAAEPLASPVNTTSDDPGFWLYSSGSTG
KPKGTVHTHANLWWTAELYGKPVLALTEDDVCFSAAKMYFAYGLGNALTFPLSVGATVVL
MAERPTPDATFRRWMEHKPTVFFGAPTGFAGMLASPKLPAREAVALRMCSSAGEALPGEI
AQRFKAQFGCEIIDGIGSTEMLHIFISNRPGDIRYGTTGKPVDGYEVELRGEDGRPVPDG
EVGDLYIRGPSSALMYWTNREKSRETFQGGWTKSGDKYTRDADGYFTYAGRSDDMLKVSG
IYVSPFEVEATLMQHPSVLEAAVIGKEDTEGLTKTKAFVVLKDGAAASEEELKAFVKERL
APYKYPRFLEFVSELPKTATGKIQRFRLREKEKQA