Protein Info for PS417_17365 in Pseudomonas simiae WCS417

Updated annotation (from data): galactaro-1,5-lactonase
Rationale: Specifically important in carbon source D-Galacturonic Acid monohydrate. This is the second step after D-galacturonate dehydrogenase (PS417_17360) and forms meso-galactarate, which is the substrate of PS417_04210. The substrate is probably the 1,5-lactone, not the 1,4-lactone, given the characterization of the D-galacturonate dehydrogenase from Agrobacterium tumefaciens (PMID:24450804), which is 51% identical to PS417_17360.
Original annotation: gluconolactonase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08450: SGL
  • amino acids 13 to 261 (249 residues), 275.6 bits, E=2.1e-86

Best Hits

KEGG orthology group: None (inferred from 93% identity to pfs:PFLU3950)

Predicted SEED Role

"Gluconolactonase (EC 3.1.1.17)" in subsystem Entner-Doudoroff Pathway (EC 3.1.1.17)

MetaCyc Pathways

• glucose degradation (oxidative) (5/5 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
• glucose and glucose-1-phosphate degradation (4/5 steps found)
• L-ascorbate biosynthesis VIII (engineered pathway) (5/7 steps found)
• sorbitol biosynthesis II (2/3 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)
• L-ascorbate biosynthesis IV (animals, D-glucuronate pathway) (3/6 steps found)
• L-ascorbate biosynthesis VI (plants, myo-inositol pathway) (1/4 steps found)

KEGG Metabolic Maps

• Ascorbate and aldarate metabolism
• Caprolactam degradation
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.17

Use Curated BLAST to search for 3.1.1.17

Sequence Analysis Tools

See A0A1N7TYW3 at UniProt or InterPro

Protein Sequence (291 amino acids)

>PS417_17365 galactaro-1,5-lactonase (Pseudomonas simiae WCS417)
MNAELIVDARNAVGECPVWVPGENALYWVDIPKGGLQRWSAATGHVAAWTAPQMLACIAR
TDAGNWVAGMETGFFQLTPHNDGSLDTTLLAAVEHPRQDMRLNDGRCDRQGRFWAGSMVL
NMGLNAAEGTLYRYTSGAAPHAQLDGFITLNGLAFSPDGRTMYASDSHPLVQQIWAFDYD
IDTGTPSNRRVFVDMHKHLGRPDGAAVDADGCYWICANDAGLIHRFSPDGRLDRSLTVPV
KKPTMCAFGGSRLDTLFVTSIRDDQSEQSLSGGVFALNPGVVGLPEPTFTL