Protein Info for GFF3393 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 491 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 69 to 90 (22 residues), see Phobius details amino acids 207 to 231 (25 residues), see Phobius details PF00501: AMP-binding" amino acids 13 to 366 (354 residues), 197.9 bits, see alignment E=2.5e-62 PF13193: AMP-binding_C" amino acids 418 to 490 (73 residues), 65.8 bits, see alignment E=5.5e-22

Best Hits

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (491 amino acids)

>GFF3393 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTSALPTLIDLLAHNAVAHADRPAVIDDGAPQSHTMLCDAVQRQAAALSALGVGQGDRVV
LVAGNRAEVLVMLGAVAWLGAMLVPLNLRLSPAEMAQQSRDAAPVLAVVDAACAPLWQAT
QAAWPEGMPGVPVVALGAANWPSLACAGPTTAPPVARQDAPALGVLMLFTAAVEGHARGA
VLAQSQLTASARQIGEVWSLGADDRWLGVLPLFHAAGIGLSLALLAAGGTCVLLPRFDPA
AAAAAVEQHQVTVSATFAPMLGALLDAAQASGASLASLRIGMGLEPPATQARLAALCPGA
GFWSAYGQAEVSSMVCLGLQSERPGAAGRAVPPSQIRIQDAAGQPVATGMEGEIAVRGPT
VFLGYWDAAQQRPFKPADPWHRTGDLGRLDEDGWLWFTGRAAHKQLIKSGGENVYPAEVE
QVLLEHPAVAEAYVFGQPDEKWGEAVHAACALRAGATATEAELIAFVEQRLARYKRPQRV
RLQAAPLQRSA