Protein Info for GFF3391 in Pseudomonas sp. DMC3

Annotation: Phosphinothricin N-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 183 PF13302: Acetyltransf_3" amino acids 3 to 138 (136 residues), 29.4 bits, see alignment E=2.9e-10 PF13420: Acetyltransf_4" amino acids 5 to 155 (151 residues), 81.4 bits, see alignment E=1.9e-26 PF13673: Acetyltransf_10" amino acids 39 to 141 (103 residues), 30.4 bits, see alignment E=8.8e-11 PF00583: Acetyltransf_1" amino acids 51 to 137 (87 residues), 54.6 bits, see alignment E=3.3e-18 PF13508: Acetyltransf_7" amino acids 54 to 138 (85 residues), 32.5 bits, see alignment E=2.3e-11

Best Hits

KEGG orthology group: K03823, phosphinothricin acetyltransferase [EC: 2.3.1.183] (inferred from 66% identity to pfv:Psefu_1258)

MetaCyc: 43% identical to demethyl-phosphinothricin N-acetyltransferase (Kitasatospora phosalacinea)
Phosphinothricin acetyltransferase. [EC: 2.3.1.183]

Predicted SEED Role

"phosphinothricin N-acetyltransferase, putative"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.183

Use Curated BLAST to search for 2.3.1.183

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (183 amino acids)

>GFF3391 Phosphinothricin N-acetyltransferase (Pseudomonas sp. DMC3)
MALKIRTATVADAVAIQRIYAPIVSTTAISFEEIPPSAEEIAQRIATTLLTYPYLVAEEG
GEIKGYAYASQHRARPAYRWAVDVTVYIAESARRQGVGRELYETLLPILEKQRFRAAYAG
VAQPNEGSVGLHESLGFVHIGTYPEVGFKLGKWHDVGYWRLGLCQATPPLEPILFPKLEV
SSQ