Protein Info for PS417_17310 in Pseudomonas simiae WCS417

Annotation: allantoate amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 18 to 414 (397 residues), 449.7 bits, see alignment E=4.5e-139 PF04389: Peptidase_M28" amino acids 72 to 179 (108 residues), 32.3 bits, see alignment E=1.2e-11 PF01546: Peptidase_M20" amino acids 89 to 413 (325 residues), 105.4 bits, see alignment E=6.1e-34 PF07687: M20_dimer" amino acids 221 to 321 (101 residues), 28.2 bits, see alignment E=2.3e-10

Best Hits

Swiss-Prot: 47% identical to HYUC_RHIML: N-carbamoyl-L-amino-acid hydrolase (hyuC) from Rhizobium meliloti

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 97% identity to pfs:PFLU3940)

MetaCyc: 47% identical to N-carbamoyl-L-amino-acid hydrolase subunit (Sinorhizobium meliloti CECT4114)
N-carbamoyl-L-amino-acid hydrolase. [EC: 3.5.1.87]

Predicted SEED Role

"Beta-ureidopropionase (EC 3.5.1.6)" in subsystem Hydantoin metabolism or L-2-amino-thiazoline-4-carboxylic acid-Lcysteine conversion or Pyrimidine utilization (EC 3.5.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.87

Use Curated BLAST to search for 3.5.1.6 or 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UN09 at UniProt or InterPro

Protein Sequence (427 amino acids)

>PS417_17310 allantoate amidohydrolase (Pseudomonas simiae WCS417)
MNAALDVLQSTHQHINRDRLWQSLMDLAKLGATPKGGVCRLALTDLDRKARDLFVKWCEE
AGCTVTIDGIGNIFARRPGRNPNLPPVMTGSHIDTQPTGGKFDGCFGVLAGVEVLRTLND
LKVETEAPLEVVVWTNEEGSRFPPCMMGSGVFAEKFTLADTLAKVDVDGVSVGDALNAIG
YAGSRAVSGHKVGAYFEAHIEQGPILEDEQKTIGVVLGALGQKWFDLKLHGVEAHAGPTP
MHLRKDALVGAAAVVAAVNAAALGHQPHACGTVGCLQAYPGSRNVIPGEVRMTLDFRHLE
PARLDSMIAQVRKVIEDTCAKHGLSFEMEPTADFPPLYFDKGCVDAVRNAANGLGLSNMD
IVSGAGHDAIFVAELGPAGMIFVPCEGGISHNEIENAAPDDLAAGCAVLLRAMVAASAAI
ANGKLAA