Protein Info for PS417_01720 in Pseudomonas simiae WCS417

Updated annotation (from data): Urocanate hydratase (EC 4.2.1.49)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (UROCANATE-HYDRATASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: urocanate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01228: urocanate hydratase
  • amino acids 11 to 554 (544 residues), 1017.2 bits, E=0
• PF17391: Urocanase_N
  • amino acids 12 to 138 (127 residues), 206.4 bits, E=1.6e-65
• PF01175: Urocanase
  • amino acids 141 to 349 (209 residues), 296.9 bits, E=1.2e-92
• PF17392: Urocanase_C
  • amino acids 352 to 546 (195 residues), 311.5 bits, E=3e-97

Best Hits

Swiss-Prot: 99% identical to HUTU_PSEFS: Urocanate hydratase (hutU) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 99% identity to pfs:PFLU0361)

MetaCyc: 91% identical to urocanase subunit (Pseudomonas putida)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

See A0A1N7U5P9 at UniProt or InterPro

Protein Sequence (556 amino acids)

>PS417_01720 Urocanate hydratase (EC 4.2.1.49) (Pseudomonas simiae WCS417)
MTKPTKYRDVEIRAAHGNKLTAKSWLTEAPLRMLMNNLDPQVAENPKELVVYGGIGRAAR
NWECYDQIVESLTHLNDDETLLVQSGKPVGVFKTHSNAPRVLIANSNLVPHWASWEHFNE
LDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVEAGRQHYNSDLTGRWVLTAGLGGMGG
AQPLAATLAGACSLNIECQQVSIDFRLNSRYVDEQATDLDDALARIAKYTKEGKAISVAL
LGNAAEILPELVKRGVRPDMVTDQTSAHDPLNGYLPAGWTWDEYRARAKTEPAAVIKAAK
QSMAVHVKAMLEFQKQGIPTFDYGNNIRQMAQEEGVENAFDFPGFVPAYIRPLFCRGIGP
FRWAALSGDPQDIYKTDAKVKELIPDDAHLHNWLDMARERISFQGLPARICWVGLGQRAK
LGLAFNEMVRSGELSAPVVIGRDHLDSGSVASPNRETEAMQDGSDAVSDWPLLNALLNTA
SGATWVSLHHGGGVGMGFSQHSGMVIVCDGTDEAAERIARVLHNDPATGVMRHADAGYQI
AIDCAKEQGLNLPMIK