Protein Info for GFF3377 in Xanthobacter sp. DMC5

Annotation: Cation/acetate symporter ActP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 539 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 35 to 57 (23 residues), see Phobius details amino acids 79 to 101 (23 residues), see Phobius details amino acids 107 to 125 (19 residues), see Phobius details amino acids 151 to 172 (22 residues), see Phobius details amino acids 184 to 204 (21 residues), see Phobius details amino acids 215 to 235 (21 residues), see Phobius details amino acids 265 to 289 (25 residues), see Phobius details amino acids 301 to 326 (26 residues), see Phobius details amino acids 362 to 387 (26 residues), see Phobius details amino acids 408 to 427 (20 residues), see Phobius details amino acids 433 to 457 (25 residues), see Phobius details amino acids 465 to 485 (21 residues), see Phobius details amino acids 503 to 521 (19 residues), see Phobius details TIGR00813: transporter, solute:sodium symporter (SSS) family" amino acids 66 to 472 (407 residues), 192.1 bits, see alignment E=8.8e-61 PF00474: SSF" amino acids 66 to 472 (407 residues), 333.1 bits, see alignment E=1.3e-103

Best Hits

Swiss-Prot: 57% identical to ACTP_ERWT9: Cation/acetate symporter ActP (actP) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K14393, cation/acetate symporter (inferred from 82% identity to xau:Xaut_0986)

MetaCyc: 55% identical to acetate/glycolate:cation symporter (Escherichia coli K-12 substr. MG1655)
RXN0-1981; RXN0-5111; TRANS-RXN0-576

Predicted SEED Role

"Acetate permease ActP (cation/acetate symporter)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (539 amino acids)

>GFF3377 Cation/acetate symporter ActP (Xanthobacter sp. DMC5)
MTIDKLFPTAFLALPALLFAGPAMAAGAAGGGGSNATAISMFVAIVLVTLCITAWAARRT
KSTSDFYAAGGHISGFQNGVALTGDMVSAGALLGLAGLVFASGFDGLVFAVGYSTGLPLV
VFLVAERLRKLGRFTVADVLSTRLEERSIRIFTAFATLVIVIFYLIAQMVGAGQLIRLLF
GLDYIYAQILVGALMICYVMFGGMMATTWVQIIKALLLIGAAVFIAIAVLAHFGFSYNDM
VARAVSVHPRGEAMMLPKVFSGGPLQTISLGLTLMLGTAGLPHVLMRFFTVPDTRAARVS
VFWGTLGVSGFFALIFVIGFGALAIIGGDPAYVDANGALRGGGNMATIHLAHAIGGDPLM
GFVSAVAFATILAVVAGLTLAGASAVSHDLYARALCRGAVSEAREIRVSRIATLVLGVIS
VLLGIVFQTQNVAYMISLAYAIACSSTLPLLILTLYWSGLTTRGAITGGAVGLTAAVALT
VLGPPVWVKVLGNATPIFPIDPPTIVSMPLAFFTAWLVSVLDRRAREGVGMGGGLEAAE