Protein Info for GFF3376 in Variovorax sp. SCN45

Annotation: Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 transmembrane" amino acids 215 to 232 (18 residues), see Phobius details PF00501: AMP-binding" amino acids 22 to 380 (359 residues), 249.2 bits, see alignment E=6.2e-78 PF13193: AMP-binding_C" amino acids 431 to 506 (76 residues), 84.2 bits, see alignment E=1e-27

Best Hits

KEGG orthology group: None (inferred from 67% identity to rer:RER_46870)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (518 amino acids)

>GFF3376 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) (Variovorax sp. SCN45)
LHSPPATASPARGPTYLELIRRGALQHGQRTAIVFGDSSLTFTEVDQLSSRIAHALHAAG
ATPHTRVALLLNNGLHSVPLDFACVKAGINRVPLNSRLSLAEHARMVAETGSTHLVFGPD
LAERAAALREAVPALVCMGMGAALEGAADLLAQAGAHPAQAPAFTPSHDDVVLTLFTSGT
TGTLKAAQHTQASYAAICRNVLLNLLPAAPDDAMLHAASLIHASGVFVLPFWLRGARTVI
LPGFEPGLFLRTLQAERITAINLVPTMLQMLFEHPGIATTDVAALRYVIYGASPMPRPVI
QRAMAAWGRHRFWQYYGQTEVPLCLAVLRPEDHTDDLLDACGQPAIDIEMRLLDEAGNEA
PHGTPGEIAVRAPSAVTGYYNAAGLNAETFTADGWVRTRDVGVFDERGFLHLKDRTSDMI
ISGGYNVYPREVENALSSHPAVRECAVIGLPHEKWVEIVTAVVVLRAGQQANEAELVAHV
AGQLASYKKPQRVIFADEIPKTAVGKLNRKRLRERHRD