Protein Info for GFF3374 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00117: aconitate hydratase 2
  • amino acids 4 to 858 (855 residues), 1487.6 bits, E=0
• PF11791: Aconitase_B_N
  • amino acids 7 to 159 (153 residues), 221.1 bits, E=1e-69
• PF06434: Aconitase_2_N
  • amino acids 171 to 385 (215 residues), 310.9 bits, E=5.9e-97
• PF00330: Aconitase
  • amino acids 477 to 820 (344 residues), 135.6 bits, E=4e-43

Best Hits

Swiss-Prot: 73% identical to ACNB_SALTY: Aconitate hydratase B (acnB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01682, aconitate hydratase 2 [EC: 4.2.1.3] (inferred from 92% identity to aav:Aave_2210)

MetaCyc: 73% identical to aconitate hydratase 2 (Salmonella enterica enterica serovar Typhimurium)
2-methylisocitrate dehydratase. [EC: 4.2.1.99]; Aconitate hydratase. [EC: 4.2.1.99, 4.2.1.3]

Predicted SEED Role

"Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99)" (EC 4.2.1.3, EC 4.2.1.99)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• TCA cycle III (animals) (7/10 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• mixed acid fermentation (11/16 steps found)
• 2-methylcitrate cycle I (3/5 steps found)
• reductive TCA cycle I (7/11 steps found)
• 2-methylcitrate cycle II (3/6 steps found)
• methylaspartate cycle (12/19 steps found)
• reductive TCA cycle II (6/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3 or 4.2.1.99

Protein Sequence (866 amino acids)

>GFF3374 Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSTFLTDYRAHAAERAALGIPPLPLDAKQVADLIELIKAPPAGEDAFLLDLITHRVPPGV
DDAAKVKASFLAAVAHGDVKVGLISKSKATELLGTMVGGYNVHPLIELLDDEEVASVAAE
GLKKTLLMFDFFNDVAEKAKAGNAKARQVMQSWADAEWFTSRPEVEKTITVTVFKVPGET
NTDDLSPAPDAWSRPDIPLHYLAMLKNTREGAAFKPEEDGKRGPMQFIDDLKKKGHLVAY
VGDVVGTGSSRKSATNSVIWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGALPIE
VDVSKLEMGDVIDIHPYDGKITKGGATLVEFQLRSDVLFDEVRAGGRINLIIGRSLTAKA
REFLGLPASTVFRLPQAPAETKAGFTLAQKMVGRAVGLPEGFGVRPGTYCEPKMTTVGSQ
DTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKPVDVKTHRELPAFISNRGGVALRP
GDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFGAATGVMPLDMPESVLV
RFKGQMQPGVTLRDLVHAIPLYAIKAGLLTVAKAGKINIFSGRILEIEGLPDLKVEQAFE
LSDASAERSAAGCTIKLNPEPVKEYLTSNVVLMKNMIAQGYADARSLKRRIEKVEAWLAN
PSLLEADKDAEYAAVIEIDLADIKEPILCCPNDPDDAKTLSDVAGTKIDEAFIGSCMTNI
GHFRAAAKLLGGQRDIPVKLWVAPPTKMDESELIKEGHYSAFGTAGARTEMPGCSLCMGN
QAQVREGATVVSTSTRNFPNRLGKNTNVYLASAELAAIASKLGHIPTVAEYHEAMGVVNK
DGAAIYQYMNFDQIEEYAEAAKGVTA