Protein Info for GFF3373 in Variovorax sp. SCN45

Annotation: Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00532: hydroxymethylglutaryl-CoA reductase, degradative
  • amino acids 3 to 386 (384 residues), 434.1 bits, E=2.9e-134
• PF00368: HMG-CoA_red
  • amino acids 16 to 386 (371 residues), 371.2 bits, E=3e-115

Best Hits

Swiss-Prot: 74% identical to MVAA_PSEMV: 3-hydroxy-3-methylglutaryl-coenzyme A reductase (mvaA) from Pseudomonas mevalonii

KEGG orthology group: K00054, hydroxymethylglutaryl-CoA reductase [EC: 1.1.1.88] (inferred from 97% identity to vpe:Varpa_0083)

MetaCyc: 80% identical to hydroxymethylglutaryl-CoA reductase (Delftia acidovorans SPH-1)
Hydroxymethylglutaryl-CoA reductase. [EC: 1.1.1.88]

Predicted SEED Role

"Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34)" in subsystem Archaeal lipids or Isoprenoid Biosynthesis or Leucine Degradation and HMG-CoA Metabolism (EC 1.1.1.34)

MetaCyc Pathways

• mevalonate degradation (2/2 steps found)
• superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (6/10 steps found)
• mevalonate pathway I (eukaryotes and bacteria) (3/7 steps found)
• mevalonate pathway II (haloarchaea) (3/7 steps found)
• isoprene biosynthesis II (engineered) (3/8 steps found)
• mevalonate pathway III (Thermoplasma) (3/8 steps found)
• mevalonate pathway IV (archaea) (3/8 steps found)
• superpathway of ergosterol biosynthesis I (7/26 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)
• superpathway of cholesterol biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.34 or 1.1.1.88

Protein Sequence (429 amino acids)

>GFF3373 Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34) (Variovorax sp. SCN45)
MAVDSRIPNFRALAPAQRFAHIAQAASLTEDEVALLAGPGALSVDRANGMVENVIGTFEL
PLGVAGNFTVNGRDYLVPMAVEEPSVVAAASFMAKLAREGGGFEASSTGPLMRAQVQLIG
ITDPYGARLALLRARDEILAVANSRDKVLIGLGGGCRDIEVHVFGDTPRGAMVVMHLIVD
VRDAMGANTVNTMAEAVSPLVEKLTGGTVRLRILSNLADLRLARARVRLAPQVLATRERS
GEEVVEGVIDAYTFAAIDPYRAATHNKGIMNGVDPVIVATGNDWRAVEAGAHAYACRGGR
YTSLTTWEKDTTGALVGTIEMPMPVGLVGGATKTHPLARLALKILGVKSAQELGEVAVAV
GLAQNLGALRALATEGIQRGHMALHARNIALVAGATGDEIDSIAKQMAAEHDVRTDRAVA
LLEALRKKK